EMCM - Capítulos de Livros

URI Permanente para esta coleçãohttps://repositorio.ufrn.br/handle/123456789/32511

Navegar

Agora exibindo 1 - 1 de 1

Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing
(Atena Editora, 2019) Santos, Yago Queiroz dos; França, Anderson Felipe Jácome de; Veras, Bruno Oliveira de; Carelli, Gabriella Silva Campos; Moura, Geovanna Maria Medeiros; Cruz, Joelton Igor Oliveira da; Oliveira, Fernanda Granja da Silva; Oliveira, João Ricardhis Saturnino de; Amorim, Luciclaudio Cassimiro de; Santos, Elizeu Antunes dos
The microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretome

SIGAA

Navegar

Navegando EMCM - Capítulos de Livros por Autor "Amorim, Luciclaudio Cassimiro de"