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Chromosome 17 abnormalities and mutation of the TP53 gene: Correlation between cytogenetics, flow cytometry and molecular analysis in three cases of chronic myeloid leukemia
(Sociedade Brasileira de Genética, 2005) Otero, Luize; Cavalcanti Júnior, Geraldo Barroso; Klumb, Claudete Esteves; Scheiner, Marcos Antonio Mauricio; Magluta, Eliane Pereira Simões; Fernandez, Teresa de Souza; Silva, Maria Luiza Macedo; Pires, Virgínia; Andrade, Gabriela Vasconcelos; Maia, Raquel Ciuvalschi; Tabak, Daniel
Alterations involving the short arm of chromosome 17 (17p) during the progression of chronic myeloid leukemia (CML) have been described. This chromosomal region contains the tumor suppressor gene TP53 that may be an important factor in the evolution of this disease. In this study, we used flow cytometry and western blotting to assess p53 protein expression and single stranded conformational polymorphism to examine TP53 gene alterations in three patients with CML who showed alterations in 17p. Only the case with del(17)(p11) had p53 expression positive by flow cytometry and an abnormal migration pattern by SSCP analysis. The importance of the correlation between the results obtained with these techniques, as well as the clinical course of the patients, are discussed.