Navegando por Autor "Castro, Aline Brito Ferreira de"
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Artigo Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury(Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2018-07) Fernandes, Aline Samaya Santos; Castro, Aline Brito Ferreira de; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Lucena, Eudes Euler de Souza; Cavalcanti, José Rodolfo Lopes de Paiva; Costa, Ianara Mendonça da; Cavalcante, Jeferson de Souza; Costa, Mirian Stela Maris de Oliveira; Guzen, Fausto PierdonáTraumatic injury to the spinal cord results in a rapid and significant loss of function. One barrier to successful regeneration in the adult CNS is the diminished axonal growth capacity after maturation. Therefore, strategies that seek to promote the restoration of function to the chronically injured spinal cord have high therapeutic value. Neurotrophic factors and peripheral nerves are known to be good substrates for bridging the lesions associatedwith CNS trauma. The role of fibroblast growth factor-2, when added to the sciatic nerve, was examined following spinal cord injury in a rat. We evaluated whether FGF-2 added to a sciatic nerve graft placed in a gap promoted nerve recovery following a complete transection of the spinal cord and if it could enhance neuronal plasticity. Rats underwent a transection at the thoracic level, which was repaired with saline or a fragment of the sciatic nerve. In another group, FGF-2 was added immediately after thelesion. The effects of FGF-2 and the fragment of the sciatic nerve graft on neuronal plasticity were investigated at the epicenterof the injury using NF-200 and 5-HT immunoreactivity after 8 weeks.A high number of NF-200 and 5-HT immunoreactive fibers were observed in the treated groups with sciatic nerve graft in the presence or absence of FGF-2 when compared to the saline group. However, a small number of NF-200(p=0.03)and 5-HT fibers were observed in the epicenter of the graft when FGF-2 was added,when compared to the group that received sciatic nerve graft. These results indicate that sciatic nerve grafting favors the growth of fibers in the traumatized spinal cord, an effect that is slightly influenced by the addition of FGF-2 to the NF-200 and 5-HT immunoreactive fibersArtigo Development of new techniques for behavioral evaluation In animals submitted to facial nerve damage(Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2019) Oliveira, Lucidio Clebeson de; Oliveira, Eligleidson José Vidal de; Lucena, Eudes Euler de Souza; Cavalcanti, José Rodolfo Lopes de Paiva; Fernandes, João Paulo Costa; Souza Júnior, José Edvan de; Cavalcante, Jeferson de Souza; Azevedo, Eduardo Pereira de; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Castro, Aline Brito Ferreira de; Guzen, Fausto PierdonáThree new methods for evaluating facial nerve injury were developed: Spontaneous ocular opening, vibrissae reflex and resistance to upper eyelid lifting. Nerve crush injury was performed by pressing the mice’s facial nerve with forceps for 30 seconds. Scores were given based on the comparison between the behavioral assessment of the injured and non-injured sides. Facial nerve injury is currently evaluated by observing the movement of the mice’s vibrissae and the ocular closure. In this study, three additional tests are proposed. Therefore, a wider evaluation can be accomplished where all the aspects of the facial mimicry can be assessed. The use of the 3 new methods described in this study, in addition to the 2 methods currently used, allows a complete behavioral assessment of facial nerve lesions as several behavioral aspects related to these injuries can be evaluated