Navegando por Autor "Costa, Lorenna Larissa Ferreira"
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Artigo Association of insertion-deletions polymorphisms with colorectal cancer risk and clinical features(World Journal Of Gastroenterology, 2017-10-07) Correa, Romualdo da Silva; Marques, Diego; Costa, Layse Raynara Ferreira; Costa, Lorenna Larissa Ferreira; Borges, Aline Maciel Pinheiro; Ito, Fernanda Ribeiro; Ramos, Carlos Cesar de Oliveira; Bortolin, Raul Hernandes; Luchessi, André Ducati; Santos, Ândrea Ribeiro dos; Santos, Sidney; Silbiger, Vivian NogueiraAIM To investigate the association between 16 insertion-deletions (INDEL) polymorphisms, colorectal cancer (CRC) risk and clinical features in an admixed population. Methodos: One hundred and forty patients with CRC and 140 cancer-free subjects were examined. Genomic DNA was extracted from peripheral blood samples. Polymorphisms and genomic ancestry distribution were assayed by Multiplex-PCR reaction, separated by capillary electrophoresis on the ABI 3130 Genetic Analyzer instrument and analyzed on GeneMapper ID v3.2. Clinicopathological data were obtained by consulting the patients’ clinical charts, intra-operative documentation, and pathology scoring. Rerults: Logistic regression analysis showed that polymorphism variations in IL4 gene was associated with increased CRC risk, while TYMS and UCP2 genes were associated with decreased risk. Reference to anatomical localization of tumor Del allele of NFKB1 and CASP8 were associated with more colon related incidents than rectosigmoid. In relation to the INDEL association with tumor node metastasis (TNM) stage risk, the Ins alleles of ACE, HLAG and TP53 (6 bp INDEL) were associated with higher TNM stage. Furthermore, regarding INDEL association with relapse risk, the Ins alleles of ACE, HLAG, and UGT1A1 were associated with early relapse risk, as well as the Del allele of TYMS. Regarding INDEL association with death risk before 10 years, the Ins allele of SGSM3 and UGT1A1 were associated with death risk. Conclusion: The INDEL variations in ACE, UCP2, TYMS, IL4, NFKB1, CASP8, TP53, HLAG, UGT1A1, and SGSM3 were associated with CRC risk and clinical features in an admixed population. These data suggest that this cancer panel might be useful as a complementary tool for better clinical management, and more studies need to be conducted to confirm these findings.TCC A bioespectroscopia na medicina personalizada(Universidade Federal do Rio Grande do Norte, 2019-11-14) Costa, Lorenna Larissa Ferreira; Lajus, Tirzah Braz Petta; Lima, Kassio Michell Gomes de; Timóteo, Ana Rafaela de Souza; Gavioli, Elaine CristinaA bioespectroscopia permite realizar a análise de biofluidos fornecendo a caracterização completa de todas as moléculas presentes na matriz biológica. Dentre as técnicas espectroscópicas, a fluorescência molecular se destaca quanto a sua elevada sensibilidade analítica na ampla identificação das informações obtidas pela matriz de fluorescência excitação-emissão (EEM), permitindo a medição específica de espécies fluorescentes na amostra. Pacientes com câncer de mama portadoras de alterações polimórficas no gene CYP2D6, apresentam variações consideráveis na biodisponibilidade do tamoxifeno (TMX), em decorrência da necessidade de bioativação desse fármaco, consequentemente, pacientes com tais alterações alélicas apresentam impacto negativo no seu prognóstico. A quantificação do TMX através da espectroscopia de fluorescência fornece uma avaliação fenotípica do status metabolizador farmacológico dessa medicação pela paciente, permitindo conduzi-la para uma terapia farmacológica personalizada e eficaz. De acordo com os resultados obtidos, a fluorescência molecular foi capaz de detectar os sinais fluorescentes gerados pela estrutura de TMX em solução de plasma através da metodologia do padrão interno. Apesar das interferências de sinal em decorrência da vasta variedade molecular presente nessa matriz, algoritmos estatísticos multivariados permitiram extrair informações relevantes do analito, melhorando o sinal/ruído da matriz de emissão-excitação. Dessa maneira, a detecção e a quantificação do fármaco TMX em amostras de plasma sanguíneo através do espectrofluorímetro demonstra a eficiência dessa técnica na análise deste fármaco em matriz biológica, apresentando baixo custo na aquisição instrumental e agilidade na execução, viabilizando-o como ótima estratégia auxiliar para o acompanhamento personalizado de pacientes na rotina clínica.Artigo Role of miRNAs in sigmoid colon cancer: a search for potential biomarkers(MDPI, 2020-11-10) Correa, Romualdo da Silva; Marques, Diego; Costa, Layse Raynara Ferreira; Costa, Lorenna Larissa Ferreira; Oliveira, Ana Beatriz Bezerra; Ramos, Carlos Cesar de Oliveira; Sandoval, Tatiana Vinasco; Lopes, Katia de Paiva; Vialle, Ricardo Assunção; Vidal, Amanda Ferreira; Silbiger, Vivian Nogueira; Santos, Ândrea Ribeiro dosThe aberrant expression of microRNAs in known to play a crucial role in carcinogenesis. Here, we evaluated the miRNA expression profile of sigmoid colon cancer (SCC) compared to adjacent-to-tumor (ADJ) and sigmoid colon healthy (SCH) tissues obtained from colon biopsy extracted from Brazilian patients. Comparisons were performed between each group separately, considering as significant p-values < 0.05 and |Log2(Fold-Change)| > 2. We found 20 differentially expressed miRNAs (DEmiRNAs) in all comparisons, two of which were shared between SCC vs. ADJ and SCC vs. SCH. We used miRTarBase, and miRTargetLink to identify target-genes of the differentially expressed miRNAs, and DAVID and REACTOME databases for gene enrichment analysis. We also used TCGA and GTEx databases to build miRNA-gene regulatory networks and check for the reproducibility in our results. As findings, in addition to previously known miRNAs associated with colorectal cancer, we identified three potential novel biomarkers. We showed that the three types of colon tissue could be clearly distinguished using a panel composed by the 20 DEmiRNAs. Additionally, we found enriched pathways related to the carcinogenic process in which miRNA could be involved, indicating that adjacent-to-tumor tissues may be already altered and cannot be considered as healthy tissues. Overall, we expect that these findings may help in the search for biomarkers to prevent cancer progression or, at least, allow its early detection, however, more studies are needed to confirm our results.