Navegando por Autor "Cruz, Luis J."

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Biological evaluations of nanocarriers to improve the effectiveness of colorectal cancer treatment
(Universidade Federal do Rio Grande do Norte, 2022-03-24) Oliveira, Ana Luiza Cabral de Sá Leitão; Araújo Júnior, Raimundo Fernandes de; http://lattes.cnpq.br/1903940945895093; http://lattes.cnpq.br/6353685475398176; Gasparotto, Luiz Henrique da Silva; https://orcid.org/0000-0002-4711-393X; http://lattes.cnpq.br/2649423643904707; Cruz, Luis J.; Geus-oei, Lioe-fee de; Leitão, Renata Ferreira de Carvalho
Um dos desafios da nanotecnologia é melhorar a eficácia dos tratamentos de doenças, a fim de reduzir as taxas de morbimortalidade. Seguindo essa linha de estudo, fizemos uma formulação de nanopartículas com tamanho pequeno, superfícies uniformes e coeficiente de encapsulamento satisfatório como alvo para células de câncer colorretal. Os resultados de ligação e absorção provam que o uso do sistema alvo com ácido fólico funciona: Usando este sistema, a citotoxicidade e a morte celular são aumentadas quando comparadas ao uso de oxaliplatina livre. Os dados mostram que o sistema maximizou a eficiência da oxaliplatina na modulação da progressão tumoral, aumentando a apoptose e diminuindo a resistência à droga. Assim, pela primeira vez, nossos achados sugerem que o PLGA-PEG-FA aumenta a eficácia antitumoral da oxaliplatina ao funcionar como um facilitador da liberação de drogas no câncer colorretal.
Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defect increased FGF-2, osteocalcin and OPG immunostaining and IL-10 levels
(American Journal of Translational Research, 2020-01-15) Vasconcelos, Roseane Carvalho; Ferreira, Camyla; Araújo, Eduarda Medeiros de; Motta, Fabiana Villela da; Delmonte, Mauricio Roberto Bomio; Araújo Júnior, Raimundo Fernandes de; Paiva, Daniel Felipe Fernandes; Pirih, Flavia Q.; Silva, José Sandro Pereira da; Chan, Alan B.; Cruz, Luis J.; Ishii, Makiko; Medeiros, Caroline Addison Carvalho Xavier de; Guerra, Gerlane Coelho Bernardo; Araújo, Aurigena Antunes de
The aim of this study was to characterize and evaluate zirconia/hydroxyapatite in a critical size calvarial defect model in rats. Zirconia/hydroxyapatite (80/20) scaffold was characterized by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). Critical size (8 mm) calvarial defects were created in wistar rats (n=48) and divided into four groups (90 days): G0 Group: positive control; G1 Group: hydroxyapatite; G2 Group: Zirconia; G3 Group: Zirconia/hydroxyapatite (80/20). Calvaria were subjected to Micro CT, histological and immunohistochemical analyses (RANK, RANKL, OPG, osteocalcin and FGF-2). IL-1 beta, IL-10 and TNF-alpha levels were analyzed by Elisa Immunoassay. The XRD analysis confirmed the formation of a crystalline structure and SEM showed the presence of regions corresponding to Zirconia and Hydroxyapatite. The Micro CT showed increased bone volume (BV/TV) and bone mineral density (BMD) in the G3 group (P<0.05). In addition, discrete periosteal bone formation was found at the interface of the defect edge and the external surface of the scaffold in the G3 group, showing osteocytes inside and osteoblasts (P<0.05) with scarce mononuclear inflammatory cells (P<0.01) in the central region of the defect. The immunostaining was moderate for RANKL, Osteocalcin and FGF-2 in the G3 group (P<0.5), while it was intense for OPG (P<0.001). IL-1 beta levels were decreased and IL-10 levels increased (P<0.05). Zirconia/hydroxyapatite (80/20) scaffold repair in critical size calvarial defects increased bone density, osteoblast and osteoclast cell numbers, FGF-2, osteocalcin and OPG immunostaining and IL-10 levels

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Navegando por Autor "Cruz, Luis J."