Navegando por Autor "Galvão, Ana Cecília de Menezes"

Agora exibindo 1 - 12 de 12

Changes in cortisol but not in brain-derived neurotrophic factor modulate the association between sleep disturbances and major depression
(2020-04-28) Santiago, Giuliana Travassos Pires; Galvão, Ana Cecília de Menezes; Almeida, Raíssa Nóbrega de; Mota-Rolim, Sergio Arthuro; Palhano-Fontes, Fernanda; Maia-de-Oliveira, João Paulo; Araújo, Dráulio Barros de; Lobão-Soares, Bruno; Galvão-Coelho, Nicole Leite
Sleep disturbance is a symptom consistently found in major depression and is associated with a longer course of illness, reduced response to treatment, increased risk of relapse and recurrence. Chronic insomnia has been associated with changes in cortisol and serum brain-derived neurotrophic factor (BDNF) levels, which in turn are also changed in major depression. Here, we evaluated the relationship between sleep quality, salivary cortisol awakening response (CAR), and serum BDNF levels in patients with sleep disturbance and treatment-resistant major depression (n = 18), and in a control group of healthy subjects with good (n = 21) and poor (n = 18) sleep quality. We observed that the patients had the lowest CAR and sleep duration of all three groups and a higher latency to sleep than the healthy volunteers with a good sleep profile. Besides, low CAR was correlated with more severe depressive symptoms and worse sleep quality. There was no difference in serum BDNF levels between groups with distinct sleep quality. Taken together, our results showed a relationship between changes in CAR and in sleep quality in patients with treatment-resistant depression, which were correlated with the severity of disease, suggesting that cortisol could be a physiological link between sleep disturbance and major depression.
Common marmosets: a potential translational animal model of juvenile depression
(2017-09-21) Galvão-Coelho, Nicole Leite; Galvão, Ana Cecília de Menezes; Silva, Flávia Santos da; Sousa, Maria Bernardete Cordeiro de
Major depression is a psychiatric disorder with high prevalence in the general population, with increasing expression in adolescence, about 14% in young people. Frequently, it presents as a chronic condition, showing no remission even after several pharmacological treatments and persisting in adult life. Therefore, distinct protocols and animal models have been developed to increase the understanding of this disease or search for new therapies. To this end, this study investigated the effects of chronic social isolation and the potential antidepressant action of nortriptyline in juvenile Callithrix jacchus males and females by monitoring fecal cortisol, body weight, and behavioral parameters and searching for biomarkers and a protocol for inducing depression. The purpose was to validate this species and protocol as a translational model of juvenile depression, addressing all domain criteria of validation: etiologic, face, functional, predictive, inter-relational, evolutionary, and population. In both sexes and both protocols (IDS and DPT), we observed a significant reduction in cortisol levels in the last phase of social isolation, concomitant with increases in autogrooming, stereotyped and anxiety behaviors, and the presence of anhedonia. The alterations induced by chronic social isolation are characteristic of the depressive state in non-human primates and/or in humans, and were reversed in large part by treatment with an antidepressant drug (nortriptyline). Therefore, these results indicate C. jacchus as a potential translational model of juvenile depression by addressing all criteria of validation.
Effect of chronic stress during adolescence in prefrontal cortex structure and function
(2017) Folha, Otávio Augusto de Araújo Costa; Bahia, Carlomagno Pacheco; Aguiar, Gisele Priscila Soares de; Herculano, Anderson Manoel; Coelho, Nicole Leite Galvão; Sousa, Maria Bernardete Cordeiro de; Shiramizu, Victor Kenji Medeiros; Galvão, Ana Cecília de Menezes; Carvalho, Walther Augusto de; Pereira, Antonio
Critical periods of plasticity (CPPs) are defined by developmental intervals wherein neuronal circuits are most susceptible to environmental influences. The CPP of the prefrontal cortex (PFC), which controls executive functions, extends up to early adulthood and, like other cortical areas, reflects the maturation of perineuronal nets (PNNs) surrounding the cell bodies of specialized inhibitory interneurons. The aim of the present work was to evaluate the effect of chronic stress on both structure and function of the adolescent’s rat PFC. We subjected P28 rats to stressful situations for 7, 15 and 35 days and evaluated the spatial distribution of histochemically-labeled PNNs in both the Medial Prefrontal Cortex (MPFC) and the Orbitofrontal Cortex (OFC) and PFC-associated behavior as well. Chronic stress affects PFC development, slowing PNN maturation in both the (MPFC) and (OFC) while negatively affecting functions associated with these areas. We speculate upon the risks of prolonged exposure to stressful environments in human adolescents and the possibility of stunted development of executive functions.
Endocrine and Cognitive Adaptations to Cope with Stress in Immature Common Marmosets (Callithrix jacchus): Sex and Age Matter
(2015-11-30) Sousa, Maria Bernardete Cordeiro de; Galvão, Ana Cecília de Menezes; Sales, Carla Jéssica Rodrigues; Castro, Dijenaide Chaves de; Galvão-Coelho, Nicole Leite Galvão-Coelho
Phenotypic sex differences in primates are associated with body differentiation during the early stages of life, expressed in both physiological and behavioral features. Hormones seem to play a pivotal role in creating a range of responses to meet environmental and social demands, resulting in better reactions to cope with challenges to survival and reproduction. Steroid hormones actively participate in neuroplasticity and steroids from both gonads and neurons seem to be involved in behavioral modulation in primates. Indirect evidence suggests the participation of sexual steroids in dimorphism of the stress response in common marmosets. This species is an important experimental model in psychiatry, and we found a dual profile for cortisol in the transition from juvenile to subadult, with females showing higher levels. Immature males and females at 6 and 9 months of age moved alone from the family group to a new cage, over a 21-day period, expressed distinct patterns of cortisol variation with respect to range and duration of response. Additional evidence showed that at 12 months of age, males and females buffered the hypothalamic-pituitary-adrenal axis during chronic stress. Moreover, chronic stressed juvenile marmoset males showed better cognitive performance in working memory tests and motivation when compared to those submitted to short-term stress living in family groups. Thus, as cortisol profile seems to be sexually dimorphic before adulthood, age and sex are critical variables to consider in approaches that require immature marmosets in their experimental protocols. Moreover, available cognitive tests should be scrutinized to allow better investigation of cognitive traits in this species.
Investigação de potenciais biomarcadores da depressão maior
(Universidade Federal do Rio Grande do Norte, 2021-04-07) Galvão, Ana Cecília de Menezes; Coelho, Nicole Leite Galvão; Coelho, Nicole Leite Galvão; 00847910474; http://lattes.cnpq.br/9256395169042054; http://lattes.cnpq.br/9256395169042054; http://lattes.cnpq.br/5268464162790396; Baes, Cristiane Von Werne; http://lattes.cnpq.br/1322121837170202; Barbosa, Flavio Freitas; http://lattes.cnpq.br/3611261597113820; Tofoli, Luís Fernando Farah de; http://lattes.cnpq.br/5025236380342711; Sousa, Maria Bernardete Cordeiro de; http://lattes.cnpq.br/8488760386226790
A compreensão da fisiopatologia das doenças psiquiátricas é mandatória para o fortalecimento da psiquiatria de precisão, auxiliando no prognóstico, diagnóstico, tratamento e acompanhamento de pacientes. Nesse contexto, o uso de biomarcadores moleculares e da qualidade do sono como ferramentas parece ser promissor. Entre as doenças psiquiátricas, os transtornos de humor, principalmente a depressão maior (TDM) tem ganhado destaque no meio científico devido à sua crescente prevalência e morbidade. Inicialmente, esse estudo avaliou a qualidade do sono (Inventário de qualidade sono de Pittsburgh: PSQI) e os níveis de diferentes moleculas periféricas: a proteína C-reativa (PCR) plasmática, o fator neurotrófico derivado do cérebro maduro sérico (mBDNF), cortisol sérico (SC) e resposta ao despertar do cortisol salivar (CAR), e os comparou entre um grupo de pacientes com transtorno depressivo maior (n = 58) e um grupo controle de voluntários saudáveis (n = 62). Enquanto os pacientes em primeiro episódio (MD n = 30) apresentaram níveis da CAR e do SC significativamente maiores do que os controles (n = 32), eles mostraram concentrações de mBDNF semelhantes. Os pacientes com depressão resistente ao tratamento (TRD n = 28) apresentaram níveis significativamente menores do SC e da CAR, e concentrações mais elevadas do mBDNF e da PCR do que os controles (n = 30). Ainda, a gravidade dos sintomas depressivos e a pior qualidade do sono foram negativamente correlacionados com SC e CAR, e positivamente para mBDNF. Em seguida, avaliamos o potencial dessas variáveis como biomarcadores de diagnóstico da TDM e de identificação dos estágios do TDM, para isso testamos alguns modelos matemáticos usando regressões logísticas multivariadas e a curva de RoC (Receiver Operating Characteristic Curve). Nenhum dos modelos de diagnóstico testados apresentou acurácia superior do que a escala de avaliação de depressão de Hamilton de 6 itens (AUC = 0,99). O melhor modelo (AUC = 0,99) que prediz os grupos de pacientes com TDM incluiu: PSQI, CAR, SC e mBDNF. Em resumo, esses achados apontam que os tipos, as intensidades e a direção das alterações biológicas no TDM diferem de acordo com os estágios da doença. Além disso, o impacto dessas alterações biológicas é principalmente relevante para predição dos grupos de pacientes com TDM, mas não para o seu diagnóstico. Assim, os presentes resultados auxiliaram na compreensão da patofisiologia do TDM e forneceram uma base que possa contribuir para estudos futuros que visem ao desenvolvimento de modelos matemáticos de biomarcadores do TDM que possam estar disponíveis comercialmente e ser utilizados na prática clínica.
Moderators of ayahuasca’s biological antidepressant action
(Frontiers Media SA, 2022-12) Sousa Júnior, Geovan Menezes de; Tavares, Vagner Deuel de Oliveira; Galvão, Ana Cecília de Menezes; Almeida, Raíssa Nóbrega de; Fontes, Fernanda Palhano Xavier de; Soares, Bruno Lobão; Freire, Fúlvio Aurélio de Morais; Nunes, Emerson Arcoverde; Oliveira, João Paulo Maia de; Perkins, Daniel; Sarris, Jerome; Araujo, Draulio Barros de; Coelho, Nicole Leite Galvão
Introduction: The understanding of biological responses to psychedelics with antidepressant potential is imperative. Here we report how a set of acute parameters, namely emotional (depressive symptoms), cognitive (psychedelic experience), and physiological (salivary cortisol), recorded during an ayahuasca dosing session, modulated serum brain-derived neurotrophic factor (BDNF), serum cortisol (SC), serum interleukin 6 (IL-6), plasma C-reactive protein (CRP), and salivary cortisol awakening response (CAR). Methods: Results were analyzed 2 days after the psychedelic intervention (ayahuasca) versus placebo in both patients with treatment-resistant depression and healthy volunteers. These measures were assessed as part of a randomized double-blinded, placebo-controlled trial (n = 72). Results: Results revealed that larger reductions of depressive symptoms during the dosing session significantly moderated higher levels of SC in patients. Whereas lesser changes in salivary cortisol levels during the ayahuasca intervention were related to higher BDNF levels in patients with a larger clinical response in the reduction in depressive symptoms. No moderator was found for patient's CAR, IL-6, and CRP responses to ayahuasca and for all biomarker responses to ayahuasca in healthy controls and in the placebo group. Discussion: In summary, some specific emotional and physiological parameters during experimental ayahuasca session were revealed as critical moderators of the improvement of major depression biomarkers, mainly BDNF and SC two days after ayahuasca intake. These findings contribute to paving the way for future studies investigating the biological antidepressant response to psychedelic therapy
Modulation of serum brain-derived neurotrophic factor by a single dose of Ayahuasca: observation from a randomized controlled trial
(2019-06-04) Almeida, Raíssa Nóbrega de; Galvão, Ana Cecília de Menezes; Silva, Flávia Santos da; Silva, Erick Allan dos Santos; Palhano-Fontes, Fernanda; Maia-de-Oliveira, João Paulo; Araújo, Dráulio Barros de; Lobão-Soares, Bruno; Galvão-Coelho, Nicole Leite
Serotonergic psychedelics are emerging as potential antidepressant therapeutic tools, as suggested in a recent randomized controlled trial with ayahuasca for treatment-resistant depression. Preclinical and clinical studies have suggested that serum brain-derived neurotrophic factor (BDNF) levels increase after treatment with serotoninergic antidepressants, but the exact role of BDNF as a biomarker for diagnostic and treatment of major depression is still poorly understood. Here we investigated serum BDNF levels in healthy controls (N = 45) and patients with treatment-resistant depression (N = 28) before (baseline) and 48 h after (D2) a single dose of ayahuasca or placebo. In our sample, baseline serum BDNF levels did not predict major depression and the clinical characteristics of the patients did not predict their BDNF levels. However, at baseline, serum cortisol was a predictor of serum BDNF levels, where lower levels of serum BDNF were detected in a subgroup of subjects with hypocortisolemia. Moreover, at baseline we found a negative correlation between BDNF and serum cortisol in volunteers with eucortisolemia. After treatment (D2) we observed higher BDNF levels in both patients and controls that ingested ayahuasca (N = 35) when compared to placebo (N = 34). Furthermore, at D2 just patients treated with ayahuasca (N = 14), and not with placebo (N = 14), presented a significant negative correlation between serum BDNF levels and depressive symptoms. This is the first double-blind randomized placebo-controlled clinical trial that explored the modulation of BDNF in response to a psychedelic in patients with depression. The results suggest a potential link between the observed antidepressant effects of ayahuasca and changes in serum BDNF, which contributes to the emerging view of using psychedelics as an antidepressant. This trial is registered at http://clinicaltrials.gov (NCT02914769).
Pathophysiology of major depression by clinical stages
(Frontiers Media SA, 2021-08-05) Galvão, Ana Cecília de Menezes; Almeida, Raíssa Nobrega; Sousa Júnior, Geovan Menezes de; Miguel, Mario André Leocadio; Fontes, Fernanda Palhano Xavier de; Araujo, Draulio Barros de; Soares, Bruno Lobão; Oliveira, João Paulo Maia de; Nunes, Emerson Arcoverde; Hallak, Jaime Eduardo Cecilio; Schuch, Felipe Barreto; Sarris, Jerome; Galvão-Coelho, Nicole Leite
The comprehension of the pathophysiology of the major depressive disorder (MDD) is essential to the strengthening of precision psychiatry. In order to determine the relationship between the pathophysiology of the MDD and its clinical progression, analyzed by severity of the depressive symptoms and sleep quality, we conducted a study assessing different peripheral molecular biomarkers, including the levels of plasma C-reactive protein (CRP), serum mature brain-derived neurotrophic factor (mBDNF), serum cortisol (SC), and salivary cortisol awakening response (CAR), of patients with MDD (n = 58) and a control group of healthy volunteers (n = 62). Patients with the first episode of MDD (n = 30) had significantly higher levels of CAR and SC than controls (n = 32) and similar levels of mBDNF of controls. Patients with treatment-resistant depression (TRD, n = 28) presented significantly lower levels of SC and CAR, and higher levels of mBDNF and CRP than controls (n = 30). An increased severity of depressive symptoms and worse sleep quality were correlated with levels low of SC and CAR, and with high levels of mBDNF. These results point out a strong relationship between the stages clinical of MDD and changes in a range of relevant biological markers. This can assist in the development of precision psychiatry and future research on the biological tests for depression
Perfil de resistência bacteriana em pacientes com infecção urinária
(Universidade Federal do Rio Grande do Norte, 2024-06-25) Galvão, Ana Cecília de Menezes; Silveira, Ivanaldo Amancio da; http://lattes.cnpq.br/6992389689612013; ttp://lattes.cnpq.br/5268464162790396; Moioli, Ana Isabela Lopes Sales; https://orcid.org/0000-0001-9212-2344; http://lattes.cnpq.br/6500167286722532; Chaves, Guilherme Maranhão; https://orcid.org/0000-0002-0170-9383; http://lattes.cnpq.br/1463249528959656
A Infecção do Trato Urinário (ITU) é uma das infecções mais recorrentes no Brasil, atingindo homens e mulheres independentemente da idade. Essa infecção é caracterizada por uma inflamação do trato urinário devido ao crescimento exacerbado de bactérias oportunistas. O objetivo desse trabalho foi relatar a prevalência da resistência bacteriana nas infecções do trato urinário em pacientes atendidos no Hospital Giselda Trigueiro no período de outubro de 2022 a fevereiro de 2024. As amostras foram coletadas de pacientes internados no hospital e do ambulatório onde foram realizados a cultura e o antibiograma das amostras positivas. As amostras coletadas de origem hospitalar corresponderam a porcentagem de 85,04%das quais 30,96% foram positivas. Dessas positivas, 65,83% para bactérias e 34,17% para fungos, se verificou que o sexo feminino teve prevalência de 50,27%. As espécies mais isoladas foram a Pseudomonas aeruginosa (n = 55), Escherichia coli (n = 35), Klebsiella pneumoniae (n = 30) e Enterococcus faecalis (n = 20). Todas as cepas destas espécies isoladas apresentaram resistência a antimicrobianos com exceção as cepas de Enterococcus faecalis isoladas que não apresentaram resistência antimicrobianos testados. Pseudomonas aeruginosa, Escherichia coli e Klebsiella pneumoniae foram resistentes de uma maneira geral à ciprofloxacina. Pseudomonas aeruginosa e Klebsiella pneumoniae foram resistentes a cefepima. No ambulatório,a porcentagem de amostras coletadas foi de 14,96%. As amostras positivas foram de 30,38% das quais 97,92% para bactérias e 2,13% para fungos, verificando-se que o sexo feminino teve prevalência de 80,85%. A Escherichia coli foi à bactéria mais frequente e resistente a ceftriaxona e sulfametoxazol com trimetoprima. Verificou-se que a Escherichia coli apresentou resistência à ceftriaxona tanto no ambulatório como hospitalar. A resistência das bactérias aos antimicrobianos têm sido uma preocupação constante, pois o uso indiscriminado de antimicrobianos pode ocasionar o surgimento de bactérias multirresistentes impedindo um tratamento eficaz ou o uso de associação de vários antimicrobianos para o tratamento.
Physiological and behavioral responses to routine procedures in captive common marmosets (Callithrix jacchus)
(2016) Galvão, Ana Cecília de Menezes; Ferreira, Renata Gonçalves; Sousa, Maria Bernardete Cordeiro de; Galvão-Coelho, Nicole Leite
The effect of routine captive procedures on the welfare of species used as experimental models in biomedical research is of great interest, since stress may alter the generalization and interpretation of results. This study investigated behavioral and endocrine (fecal cortisol) reactivity patterns in common marmoset (Callithrix jacchus) adult males (N = 10) and females (N = 9) subjected to three types of routine procedures in captivity: (1) moving to a same-sized cage (P1), to a smaller cage (P2), and (2) first-time pair formation (P3). Sexually dimorphic cortisol responses were detected in animals submitted to a physical environmental stressor (cage change). Females showed an increased response throughout P1, in relation to baseline (BP) cortisol, and a trend during P2. Males increased cortisol only during P2. On the other hand, males and females showed a similar endocrine response when management involved social challenge (pair formation), with both sexes increasing cortisol levels, but females exhibited a more intense and longer-lasting cortisol increase. Males and females exhibited similar behavioral responses to cage change, except for autogrooming, with males decreasing this behavior in P1. Only females demonstrated a significantly higher increase in piloerection frequency than that of males during the pair formation phase. These endocrine and behavioral changes must be taken into account when interpreting research data that involve these types of procedures. Further studies on the impacts of routine colony management are required to devise and include protocols in official husbandry guidelines.
Potential biomarkers of major depression diagnosis and chronicity
(2021-09-29) Galvão, Ana Cecília de Menezes; Almeida, Raissa Nobrega; Sousa Júnior, Geovan Menezes de; Leocadio-Miguel, Mário André; Fontes, Fernanda Palhano Xavier de; Araujo, Draulio Barros de; Lobão-Soares, Bruno; Maia-de-Oliveira, João Paulo; Nunes, Emerson Arcoverde; Hallak, Jaime Eduardo Cecilio; Sarris, Jerome; Galvão-Coelho, Nicole Leite
Molecular biomarkers are promising tools to be routinely used in clinical psychiatry. Among psychiatric diseases, major depression disorder (MDD) has gotten attention due to its growing prevalence and morbidity. We tested some peripheral molecular parameters such as serum mature Brain-Derived Neurotrophic Factor (mBDNF), plasma C-Reactive Protein (CRP), serum cortisol (SC), and the salivary Cortisol Awakening Response (CAR), as well as the Pittsburgh sleep quality inventory (PSQI), as part of a multibiomarker panel for potential use in MDD diagnosis and evaluation of disease’s chronicity using regression models, and ROC curve. For diagnosis model, two groups were analyzed: patients in the first episode of major depression (MD: n = 30) and a healthy control (CG: n = 32). None of those diagnosis models tested had greater power than Hamilton Depression Rating Scale-6. For MDD chronicity, a group of patients with treatment-resistant major depression (TRD: n = 28) was tested across the MD group. The best chronicity model (p < 0.05) that discriminated between MD and TRD included four parameters, namely PSQI, CAR, SC, and mBDNF (AUC ROC = 0.99), with 96% of sensitivity and 93% of specificity. These results indicate that changes in specific biomarkers (CAR, SC, mBDNF and PSQI) have potential on the evaluation of MDD chronicity, but not for its diagnosis. Therefore, these findings can contribute for further studies aiming the development of a stronger model to be commercially available and used in psychiatry clinical practice
Social interactions and androgens levels in marmosets (Callithrix jacchus) in field and laboratory studies: a preliminary investigation of the Challenge Hypothesis
(2018) Sousa, Maria Bernardete Cordeiro de; Pontes, Mariana Chiste; Galvão, Ana Cecília de Menezes; Silva, Hélderes Peregrino Alves da; Galvão-Coelho, Nicole Leite
Not applicable.

SIGAA

Navegando por Autor "Galvão, Ana Cecília de Menezes"