Navegando por Autor "Guzen, Fausto Pierdoná"

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Behavioral and neurochemical effects of alpha lipoic acid associated with omega-3 in tardive dyskinesia induced by chronic haloperidol in rats
(NRC Research Press (Canadian Science Publishing), 2017-07) Araújo, Dayane Pessoa de; Camboim, Thaisa Gracielle Martins; Silva, Ana Patrícia Magalhães; Silva, Caio da Fonseca; Sousa, Rebeca Canuto de; Barbosa, Mabson Delâno Alves; Oliveira, Lucidio Clebeson; Cavalcanti, José Rodolfo Lopes de Paiva; Lucena, Eudes Euler de Souza; Guzen, Fausto Pierdoná
Tardive dyskinesia (TD) is characterized by involuntary movements of the lower portion of the face being related to typical antipsychotic therapy. TD is associated with the oxidative imbalance in the basal ganglia. Lipoic acid (LA) and omega-3 (-3) are antioxidants acting as enzyme cofactors, regenerating antioxidant enzymes. This study aimed to investigate behavioral and neurochemical effects of supplementation with LA (100 mg/kg) and -3 (1 g/kg) in the treatment of TD induced by chronic use of haloperidol (HAL) (1 mg/kg) in rats. Wistar male rats were used, weighing between 180–200 g. The animals were treated chronically (31 days) with LA alone or associated with HAL or -3. Motor behavior was assessed by open-field test, the catalepsy test, and evaluation of orofacial dyskinesia. Oxidative stress was accessed by determination of lipid peroxidation and concentration of nitrite. LA and -3 alone or associated caused an improvement in motor performance by increasing locomotor activity in the open-field test and decreased the permanence time on the bar in the catalepsy test and decreased the orofacial dyskinesia. LA and -3 showed antioxidant effects, decreasing lipid peroxidation and nitrite levels. Thus, the use of LA associated with -3 reduced the extrapyramidal effects produced by chronic use of HAL
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury
(Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2018-07) Fernandes, Aline Samaya Santos; Castro, Aline Brito Ferreira de; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Lucena, Eudes Euler de Souza; Cavalcanti, José Rodolfo Lopes de Paiva; Costa, Ianara Mendonça da; Cavalcante, Jeferson de Souza; Costa, Mirian Stela Maris de Oliveira; Guzen, Fausto Pierdoná
Traumatic injury to the spinal cord results in a rapid and significant loss of function. One barrier to successful regeneration in the adult CNS is the diminished axonal growth capacity after maturation. Therefore, strategies that seek to promote the restoration of function to the chronically injured spinal cord have high therapeutic value. Neurotrophic factors and peripheral nerves are known to be good substrates for bridging the lesions associatedwith CNS trauma. The role of fibroblast growth factor-2, when added to the sciatic nerve, was examined following spinal cord injury in a rat. We evaluated whether FGF-2 added to a sciatic nerve graft placed in a gap promoted nerve recovery following a complete transection of the spinal cord and if it could enhance neuronal plasticity. Rats underwent a transection at the thoracic level, which was repaired with saline or a fragment of the sciatic nerve. In another group, FGF-2 was added immediately after thelesion. The effects of FGF-2 and the fragment of the sciatic nerve graft on neuronal plasticity were investigated at the epicenterof the injury using NF-200 and 5-HT immunoreactivity after 8 weeks.A high number of NF-200 and 5-HT immunoreactive fibers were observed in the treated groups with sciatic nerve graft in the presence or absence of FGF-2 when compared to the saline group. However, a small number of NF-200(p=0.03)and 5-HT fibers were observed in the epicenter of the graft when FGF-2 was added,when compared to the group that received sciatic nerve graft. These results indicate that sciatic nerve grafting favors the growth of fibers in the traumatized spinal cord, an effect that is slightly influenced by the addition of FGF-2 to the NF-200 and 5-HT immunoreactive fibers
Development of new techniques for behavioral evaluation In animals submitted to facial nerve damage
(Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2019) Oliveira, Lucidio Clebeson de; Oliveira, Eligleidson José Vidal de; Lucena, Eudes Euler de Souza; Cavalcanti, José Rodolfo Lopes de Paiva; Fernandes, João Paulo Costa; Souza Júnior, José Edvan de; Cavalcante, Jeferson de Souza; Azevedo, Eduardo Pereira de; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Castro, Aline Brito Ferreira de; Guzen, Fausto Pierdoná
Three new methods for evaluating facial nerve injury were developed: Spontaneous ocular opening, vibrissae reflex and resistance to upper eyelid lifting. Nerve crush injury was performed by pressing the mice’s facial nerve with forceps for 30 seconds. Scores were given based on the comparison between the behavioral assessment of the injured and non-injured sides. Facial nerve injury is currently evaluated by observing the movement of the mice’s vibrissae and the ocular closure. In this study, three additional tests are proposed. Therefore, a wider evaluation can be accomplished where all the aspects of the facial mimicry can be assessed. The use of the 3 new methods described in this study, in addition to the 2 methods currently used, allows a complete behavioral assessment of facial nerve lesions as several behavioral aspects related to these injuries can be evaluated
Effect of FGF-2 and sciatic nerve grafting on ChAT expression in dorsal root ganglia neurons of spinal cord transected rats
(Elsevier, 2016-03) Guzen, Fausto Pierdoná; Araújo, Dayane Pessoa de; Lucena, Eudes Euler de Souza; Morais, Hécio Henrique Araújo de; Cavalcanti, José Rodolfo Lopes de Paiva; Nascimento Junior, Expedito Silva do; Costa, Miriam Stela Maris de Oliveira; Cavalcante, Jeferson de Souza
Neurotrophic factors and peripheral nerves are known to be good substrates for bridging CNS trauma. The involvement of fibroblast growth factor-2 (FGF-2) activation in the dorsal root ganglion (DRG) was examined following spinal cord injury in the rat. We evaluated whether FGF-2 increases the ability of a sciatic nerve graft to enhance neuronal plasticity, in a gap promoted by complete transection of the spinal cord. The rats were subjected to a 4 mm-long gap at low thoracic level and were repaired with saline (Saline or control group, n = 10), or fragment of the sciatic nerve (Nerve group, n = 10), or fragment of the sciatic nerve to which FGF-2 (Nerve + FGF-2 group, n = 10) had been added immediately after lesion. The effects of the FGF-2 and fragment of the sciatic nerve grafts on neuronal plasticity were investigated using choline acetyl transferase (ChAT)-immunoreactivity of neurons in the dorsal root ganglion after 8 weeks. Preservation of the area and diameter of neuronal cell bodies in dorsal root ganglion (DRG) was seen in animals treated with the sciatic nerve, an effect enhanced by the addition of FGF-2. Thus, the addition of exogenous FGF-2 to a sciatic nerve fragment grafted in a gap of the rat spinal cord submitted to complete transection was able to improve neuroprotection in the DRG. The results emphasized that the manipulation of the microenvironment in the wound might amplify the regenerative capacity of peripheral neurons
Effects of Mucuna pruriens (L.) supplementation on experimental models of parkinson's disease: a systematic review
(Medknow Publications, 2018) Idalina Neta, Francisca; Costa, Ianara Mendonça da; Lima, Francisca Overlânia Vieira; Fernandes, Luciana Cristina Borges; Cavalcanti, José Rodolfo Lopes de Paiva; Freire, Marco Aurélio de Moura; Lucena, Eudes Euler De Souza; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Azevedo, Eduardo Pereira de; Guzen, Fausto Pierdoná
Introduction: Mucuna pruriens (L.)DC. (Mp) has been used in the treatment of numerous diseases in Indian Ayurvedic medicine, mainly for delaying the symptoms of Parkinson’s disease (PD), and has as its main component levodopa (L‑DOPA). The aim of this work is to systematically review the effects of Mp supplementation on experimental models of PD due to its neuroprotective and antioxidant properties. MATERIALS AND Methods: The search was conducted through PubMed, ScienceDirect, Cochrane Library, and Scientific Electronic Library Online databases, where a number of relevant articles were found. Results: Mp demonstrated significant positive responses in the experimental models of PD by improving motor deficits and by enhancing the activity of the antioxidant systems, reducing oxidative stress. In addition, it presented some advantages when compared to a conventional antiparkinsonian drug as it minimized the occurrence and severity of dyskinesias. Conclusion: Thus, considering that the use of herbal medicines decreases the likelihood of side effects associated with the pharmaceutical drugs, this work aims to summarize and evaluate the data available regarding the mechanism of action of Mp and the reported benefits of this plant as an alternative to improve the quality of life of individuals with PD
Effects of supplementation with probiotics in experimental models of alzheimer's disease: a systematic review of animal experiments
(Bentham Science Publishers, 2022-04-06) Idalina Neta, Francisca; Souza, Francisco Emílio Simplício de; Batista, André Lima; Pinheiro, Francisco Irochima; Cobucci, Ricardo Ney; Guzen, Fausto Pierdoná
Background: Conditions along the brain-gut-microbiota (BGM) axis can significantly contribute to the pathogenesis of Alzheimer's disease (AD). Evidence from animal studies indicates a role of probiotics in regulating mood, cognition, and stress response via the BGM axis. However, the effect of probiotics on AD needs to be better clarified in preclinical and clinical studies. Methods : We prepared this systematic review according to PRISMA. PubMed, Web of Science, Embase, and Virtual Health Library (VHL) were searched for original articles concerning the effects of probiotics in experimental AD. Results: Results were presented as a narrative synthesis according to the Synthesis Without Metaanalysis (SWiM) Guideline. Seventeen studies met the inclusion criteria. The results showed significant effects in the experimental models of AD treated with probiotics alone or in mixture due to expressive improvements in cognitive tests. Conclusion: Furthermore, in most of the included studies, it was possible to observe a reduction in inflammatory processes, an increase in the concentration of peptide hormones, insulin homeostasis in the brain, increased antioxidant enzymes, and a decrease in beta-amyloid deposition and tau hyperphosphorylation. Supplementation of probiotics seems to improve performance in cognitive tests and increase the concentration of substances capable of delaying the neurodegenerative process of AD in experimental models.
Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium
(Sociedade Chilena de Anatomía, 2018-09) Lucena, Eudes Euler de Souza; Morais, Hécio Henrique Araújo de; Araújo, Dayane Pessoa de; Cavalcanti, José Rodolfo Lopes de Paiva; Azevedo, Eduardo Pereira de; Queiroz, Dinalva Brito de; Botelho, Marco Antônio; Rêgo, Amália Cinthia Meneses do; Araújo Filho, Irami; Barboza, Carlos Augusto Galvão; Nascimento Júnior, Expedito Silva do; Costa, Miriam Stela Maris de Oliveira; Cavalcante, Jeferson de Sousa; Guzen, Fausto Pierdoná
Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation
Fibrin rich in leukocytes and platelets (L-PRF) and bisphosphonate-induced osteonecrosis: we need to advertise now
(International Journal of Trend in Scientific Research and Development, 2019) Faria, Maria de Fátima; Cabral, Eric Lucas dos Santos; Castro, Wilkson Ricardo Silva; Florentino, Davidson Rogério de Medeiros; Costa Junior, João Florêncio da; Souza, Ricardo Pires de; Guzen, Fausto Pierdoná; Pinheiro, Francisco Irochima; Rêgo, Amália Cinthia Meneses; Araújo Filho, Irami
The American Association of Oral and Maxillofacial Surgeons (AAOMS) reported in 2004 a new pathology that causes osteonecrosis in the Maxillomandibular complex. The condition was named Bisphosphonate-related osteonecrosis of the jaw (BRONJ), and later renamed by medication related to jaw osteonecrosis due to the appearance of severe alveolar bone loss in the Maxillomandibular complex resulting from the use of different drugs other than Bisphosphonates. Odontology, especially oral surgery, a field concerned with newbiotechnologies, has been introducing innovations in tissue engineering. Oro-maxillofacial reconstruction is of great interest to current oral and maxillofacial surgeons, as part of the search for strategies in bioengineering and biomaterials, a major promoter of bio-dental research in our times. In order to verify the scientific evidences to analyse the effectiveness of leukocyte and platelet rich fibrin (L-PRF) on bone neoformation inMaxillomandibular complex’s surgical procedures, a search for qualified articles was conducted in the Medline (PubMed), Embase and Cochrane Central Register of Controlled Trials databases. The works analysed were published from 2013 to 2018. This review concludes that the use of platelet rich fibrin (L-PRF) is significant in the complete healing of the lesion in short postoperative periods and in the resolution of the treatments, reducing the need for postoperative re-interventions, resulting in lower morbidity for the patients
Magnetic device for closing skin wounds
(Research, Society and Development, 2020-11-11) Pinheiro, Gleyse Karina Lopes de Oliveira; Batista, André Lima; Cobucci, Ricardo Ney; Rêgo, Amália Cinthia Meneses; Araújo-Filho, Irami; Guzen, Fausto Pierdoná; Pinheiro, Francisco Irochima; https://orcid.org/0000-0001-5264-3620
The attempt to repair skin wounds dates back many years. We have observed bone fragments for making needles, hair, fibers, and animal tissues as sutures and even applying sensors to accelerate the healing process throughout history. Despite all the developments, the need for a qualified professional and prior local anesthesia to perform the suture still represent obstacles. The present study aimed to create 3D printing pieces containing N42 neodymium magnets to be fixed to the skin with adhesive tape to promote skin wounds' closure without the need for anesthesia. A descriptive, experimental study was carried out, divided into the Patent search, Ideation and creation, 3D Modeling, 3D printing of structural parts, Assembly, and Testing on artificial skin. ABSplus® plastic parts were created through 3D printing that received N42 neodymium magnets and the application of a double-sided adhesive to attach to the skin. A perilesional arrangement was simulated with the pieces created using an artificial skin model (EasySuture® Standart) after making the incision. After applying the pieces containing N42 neodymium, there was a perfect coaptation of the lesion's edges without detecting interspersed spaces in the longitudinal axis of the incision. The research resulted in creating a prototype that needs improvements and industrial adaptations for viable use in surgical practice.
Padrão de distribuição e caracterização morfológica de fibras serotonérgicas nos núcleos da linha Média/ intralaminares do tálamo do mocó (Kerodon rupestris)
(Universidade Federal do Rio Grande do Norte, 2013-03-27) Silva, Alane de Medeiros; Nascimento Júnior, Expedito Silva do; ; http://lattes.cnpq.br/5233940343676740; ; http://lattes.cnpq.br/5010602559312461; Guzen, Fausto Pierdoná; ; http://lattes.cnpq.br/7939889120780625; Cavalcante, Judney Cley;
O complexo nuclear da linha média/intralaminar forma um notável grupo de núcleos da parte medial e dorsal do tálamo. Os núcleos da linha média, em ratos, são compreendidos pelos núcleos paratenial (PT), paraventricular (PV), intermediodorsal (IMD), reuniens (Re) e rombóide (Rh). Já os intralaminares compreendem os núcleos central medial (CM), paracentral (PC), central lateral (CL) e parafascicular (PF). Tais núcleos apresentam densa inervação serotonérgica oriunda do tronco encefálico, a partir, principalmente, do denominado sistema de ativação ascendente. Esses núcleos, por sua vez, emitem projeções para diversas áreas corticais e subcorticais, mais especificamente para áreas límbicas, o que propõe o importante papel desse neurotransmissor na circuitaria límbica. O objetivo desse trabalho foi caracterizar o padrão de distribuição e morfologia das fibras de serotonérgicas nos núcleos da linha média e intralaminares do tálamo do mocó (Kerodon rupestris), um roedor típico da região Nordeste. Para isso, foram utilizados 4 mocós adultos jovens. Após etapas de perfusão e microtomia, foi realizada imunoistoquímica para serotonina (5-HT), técnica de Nissl e posterior coleta e análise de imagens a fim de caracterizar a citoarquitetura desses núcleos, bem como as fibras de 5-HT neles visualizadas. Foi realizada ainda uma análise de Densidade Óptica Relativa (DOR) para semiquantificar a concentração de fibras de 5-HT nas áreas de interesse. Sendo assim, observamos uma distribuição citoarquitetônica desses núcleos semelhante ao observado em ratos. Em se tratando da distribuição de fibras, aquelas imunorreativas a 5-HT apresentaram-se em maior concentração, conforme a DOR, nos núcleos da linha média em relação aos intralaminares, sendo o Re o núcleo que apresenta maior valor de pixels, seguido do PV, Rh, IMD e PT. Nos intralaminares o CL apresentou maior valor de pixels, seguido dos núcleos CM, PC e PF. As fibras serotonérgicas foram classificadas conforme número de varicosidades, bem como diâmetro axonal. Assim, encontramos três tipos de fibras distribuídas através desse complexo nuclear: fibras rugosas, granulares e semi-granulares. No PV predominaram fibras rugosas; PT predominaram fibras granulares; IMD, CL e PF foram representados por fibras semi-granulares e Re, Rh, PC e CM apresentaram fibras granulares e semi-granulares. A caracterização morfológica das fibras serotonérgicas encontradas e as diferenças de densidades entre os núcleos permite sugerir diferentes padrões de organização sináptica deste neurotransmissor além de confirmar seu grande repertório funcional
Plasticidade de células tronco mesenquimais de medula óssea de ratos na presença de meio condicionado do nervo facial e do fator de crescimento fibroblástico 2
(Universidade Federal do Rio Grande do Norte, 2014-06-06) Lucena, Eudes Euler de Souza; Cavalcante, Jeferson de Souza; ; http://lattes.cnpq.br/2378505945149958; ; http://lattes.cnpq.br/6969519171915250; Nascimento Júnior, Expedito Silva do; ; http://lattes.cnpq.br/5233940343676740; Guzen, Fausto Pierdoná; ; http://lattes.cnpq.br/7939889120780625; Morais, Hécio Henrique Araújo de; ; http://lattes.cnpq.br/9568109998934778; Costa, Miriam Stela Maris de Oliveira; ; http://lattes.cnpq.br/8424747565185682
Uma série de evidências mostra a influência do meio no crescimento de fibras nervosas lesadas no Sistema Nervoso Periférico (SNP), assim como o potencial do implante de Células Tronco (CTs) em tornar esse meio mais propício à regeneração nervosa. Nessa perspectiva, esse estudo teve como objetivo avaliar a plasticidade de células tronco mesenquimais (CTMs) da medula óssea de ratos diante da presença de meio de cultura condicionado com explantes de nervo facial (D-10) e fator de crescimento fibroblástico-2 (FGF-2). Dessa maneira as células mesenquimais foram cultivadadas com meio DMEM (grupo 1), só com meio D-10(grupo 2), só com FGF-2(grupo 3) ou com meio D-10 e FGF-2(grupo 4). O crescimento e a morfologia celular foram avaliados ao longo de 72 horas. Além disso, a avaliação fenotípica foi feita a partir da imunocitoquímica para GFAP, OX-42, MAP-2, β-tubulina III, NeuN e NF-200 no quarto dia de cultivo. As células cultivadas com meio condicionado sozinho ou combinado com FGF-2 demonstraram características morfológicas semelhantes a neurônios e células gliais e uma significativa atividade proliferativa nos grupos 2 e 4 ao longo dos dias. As células cultivadas com meio condicionado desprovido de tratamento com FGF-2 adquiriram fenótipo glial demostrando imunorreatividade para GFAP e OX-42. As células cultivadas com meio condicionado com adição de FGF-2 expressaram GFAP, OX-42, MAP-2, β-tubulina III, NeuN e NF-200. Em média, a área e perímetro das células do grupo 2 positivas para GFAP e a área das células imunomarcadas para OX-42 foram maiores do que as do grupo 4. O estudo possibilitou a plasticidade de células mesenquimais (CMs) numa linhagem neuronal e glial e abriu perspectivas para busca de novas técnicas com terapia e transdiferenciação celular
Projeção retiniana, caracterização citoarquitetônica e neuroquímica da zona incerta do moco (Kerodon rupestris)
(Universidade Federal do Rio Grande do Norte, 2014-03-27) Morais, Paulo Leonardo Araujo de Gois; Nascimento Júnior, Expedito Silva do; ; http://lattes.cnpq.br/5233940343676740; ; http://lattes.cnpq.br/1545549464623271; Guzen, Fausto Pierdoná; ; http://lattes.cnpq.br/7939889120780625; Cavalcante, Jeferson de Souza; ; http://lattes.cnpq.br/2378505945149958
A Zona Incerta (ZI) é um grupamento neuronal embriologicamente derivado do tálamo ventral, em continuidade com o núcleo reticular do tálamo. Diversos estudos com traçadores retrógrados e anterógrados revelaram a conexão da ZI com diversas estruturas do sistema nervoso central. Dados moleculares e citoquímicos revelaram que a ZI é um dos grupamentos neuronais com maior diversidade neuroquímica e citoarquitetônica do diencéfalo, e estudos hodológicos e neuroquímicos permitiram considerar o envolvimento da ZI em diversas funções, as quais se destacam a nocicepção, atenção, estado de alerta, controle e manutenção da postura e controle da atividade visceral. Este trabalho tem por objetivo caracterizar a citoarquitetura e o conteúdo neuroquímico da ZI do mocó (Kerodon rupestris), bem como a aferência óptica presente neste núcleo nesta espécie. A técnica de Nissl é eficiente para a delimitação e caracterização citoarquitetônica da ZI do mocó; A ZIc recebe projeção da retina contralateral, apresentando fibras Classe II ou modulator, sugerindo um caráter modulatório da informação fótica; A ZI do mocó, assim como em outros roedores e primatas, é caracterizada por uma complexa rede neuroquímica, sobretudo na porção medial da ZIr, onde encontramos imunorreatividade de todas as substâncias neuroativas investigadas, além de que A IR-NOS, GFAP e CR auxiliaram a delimitação da ZI no nível médio em ZId e ZIv. Contudo, somente fibras IR 5-HT estão presentes em todas as subdivisões da ZI. Esses dados demonstram a grande riqueza neuroquímica da ZI do mocó, auxiliando para explicar o envolvimento em um amplo repertorio funcional
State of the art in the treatment of mandibular fractures caused by firearms: case report
(Faculdade São Leopoldo Mandic, 2018-01) Souza Júnior, Erasmo Freitas de; Morais, Hécio Henrique Araújo de; Lucena, Eudes Euler de Souza; Cavalcanti, José Rodolfo Lopes de Paiva; Guzen, Fausto Pierdoná; Araújo, Dayane Pessoa de; Barbalho, Jimmy Charles Melo
Injuries to the face represent a significant risk to the health of the individual, mainly because of its significance, both functional, because it houses sensory organs and part of the respiratory and digestive systems, as well as esthetic. In this scenario, gunshot wounds in this location cause great concern on account of the magnitude of the damage, and the Oral and Maxillofacial Surgery and Traumatology team must act so that the treatment enables the rehabilitation of the patient in the shortest possible time, with a minimum of complications and sequelae. The objective of this study is to report a clinical case of a 19-year-old female patient with a comminuted fracture of the mandible body caused by a firearm projectile, treated immediately with stable internal fixation using the 2.00 mm plate-screw system for simplification of the fracture and a 2.4 mm reconstruction locking-plate on the bone gap. Relevant aspects of the surgical technique and tactics are reviewed and long-term follow-up of the patient is presented

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Navegando por Autor "Guzen, Fausto Pierdoná"