Navegando por Autor "Luchessi, Andre Ducati"
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Artigo Circulating miRNAs in acute new-onset atrial fibrillation and their target mRNA network(Wiley Online Library, 2018) Sousa, Júlio César Vieira de; Silva, Ananília Medeiros Gomes da; Araújo, Jessica Nayara Gomes de; Oliveira, Katiane Macedo de; Novaes, Ana Eloisa Melo; Lopes, Mariana Borges; Araújo Filho, Antonio Amorim de; Luchessi, Andre Ducati; Rezende, Adriana Augusto de; Hirata, Mario Hiroyuki; Silbiger, Vivian Nogueira; https://orcid.org/0000-0001-6913-4224MicroRNAs (miRNAs) are involved in the pathogenesis of atrial fibrillation (AF), acting on development and progression. Our pilot study investigated the expression of six miRNAs and their miRNA–mRNA interactions in patients with acute new-onset AF, well-controlled AF, and normal sinus rhythm (controls). Methods and results Plasma of acute new-onset AF patients (n = 5) was collected in the emergency room when patients presented with irregular and fast-atrial fibrillation rhythm. Samples from well-controlled AF (n = 16) and control (n = 15) patients were collected during medical appointments following an ECG. Expression of miR-21, miR-133a, miR-133b, miR-150, miR-328, and miR-499 was analyzed by real-time PCR. Ingenuity Pathway Analysis and the TargetScan database identified the top 30 mRNA targets of these miRNA, seeking the miRNA–mRNA interactions in cardiovascular process. Increased expression of miR-133b (1.4-fold), miR-328 (2.0-fold), and miR-499 (2.3-fold) was observed in patients with acute new-onset AF, compared with well-controlled AF and control patients. Decreased expression of miR-21 was seen in patients with well-controlled AF compared to those with acute new-onset AF and controls (0.6-fold). The miRNA-mRNA interaction demonstrated that SMAD7 and FASLG genes were the targets of miR-21, miR-133b, and miR-499 and were directly related to AF, being involved in apoptosis and fibrosis. Conclusion: The miRNAs had different expression profiles dependent on the AF condition, with higher expression in the acute new-onset AF than well-controlled AF. Clinically, this may contribute to an effective assessment for patients, leading to early detection of AF and monitoring to reduce the risk of other serious cardiovascular events.Artigo Retinol Levels and Severity of Patients with COVID-19(Nutrients, 2023) Ribeiro, Karla Danielly da Silva; Carvalho, Maria Clara da Cruz; Araujo, Júlia Kaline Carvalho Pereira; Silva, Ana Gabriella Costa Lemos da; Silva, Nayara Sousa da; Araújo, Nathalia Kelly de; Luchessi, Andre Ducati; Silbiger, Vivian Nogueira; https://orcid.org/0000-0002-2251-5967The new coronavirus infection represents a serious threat to global health and economies. In this sense, it is paramount to know the nutritional factors that may be related to the prognosis of the disease. Evidence shows that vitamin A may play an important preventive and therapeutic role in supporting respiratory infections as in COVID-19. The aim of our study was to evaluate the association of vitamin A (retinol) status with the prognosis of the disease. A case–control study from a cohort study was conducted in Brazil between May and October 2020. The study population was chosen by convenience, consisting of participants diagnosed with COVID-19. Recruitment was carried out using different approaches, including through dissemination on social media and in four hospitals in the city of Natal/RN, Brazil, recruiting participants from the COVID-19 ward and hospitalized participants who tested positive for the disease. The participants were allocated into two groups according to severity, with a group of mild (n = 88) or critical (n = 106) patients and compared to a control group (selected before the pandemic, n = 46). The extraction of retinol serum was performed and analyzed using the high-performance liquid chromatography method (HPLC). The retinol level was calculated in mmol/L, and levels below 0.7 μmol/L (20 µg/dL) were considered to be a vitamin A deficiency. Our findings suggest that the participants with mild and critical COVID-19 had lower retinol levels compared to the healthy controls (p = 0.03). In addition, milder cases of COVID-19 were associated with increased symptoms and prolonged symptoms after 90 days since the beginning of infection. However, the survival analysis showed no association with higher cases of death among participants with vitamin A deficiency (p = 0.509). More studies are needed to understand how nutritional status, including vitamin A levels, can influence prognosis and is a risk factor for the development of long COVID syndrome.