Navegando por Autor "Lyddiatt, Andrew"
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Artigo Operational intensification by direct product sequestration from cell disruptates: application of a pellicular adsorbent in a mechanically integrated disruption-fluidised bed adsorption process(Wiley, 2002-08-19) Santos, Everaldo Silvino dos; Jahanshahi, Mohsen; Sun, Yan; Pacek, Andrzej; Franco, Telma Teixera; Nienow, Alvin; Lyddiatt, AndrewA novel prototype adsorbent, designed for in-tensi®ed ¯uidised bed adsorption processes, was as-sembled by the emulsi®cation coating of 4% (w/v)porous agarose upon a zirconia-silica solid core. Theadsorbent, designated ZSA (particle density 1.75 g/ml,maximum pellicle depth 40 lm), was subjected tophysical and biochemical comparison with the perfor-mance of two commercial adsorbents (Streamline andMacrosorb K4AX). Bed expansion qualities and hydro-dynamic characteristics (N, Daxland Bo) of ZSA demon-strated a marked robustness in the face of elevatedvelocities (up to 550 cm/h) and biomass loading (up to30% (ww/v)) disrupted yeast cells. Cibracron Blue deriv-atives of the pellicular prototype (ZSA-CB), evaluated inthe batch and ¯uidised bed recovery of glyceraldehyde3-phosphate dehydrogenase (G3PDH) from unclari®edyeast disruptates, exhibited superior capacities and ad-sorption/desorption performance to the commercial derivatives. These advanced physical and biochemicalproperties facilitated a demonstration of the direct, mechanical coupling of bead-milling and ¯uidised bed ad-sorption in a fully integrated process for the acceleratedrecovery of G3PDH from yeast. The generic applicationof such pellicular adsorbents and integrated processesto the recovery of labile, intracellular products is dis-cussed