Navegando por Autor "Malfatti, Thawann"
Agora exibindo 1 - 2 de 2
- Resultados por página
- Opções de Ordenação
Artigo Anxiety-like behavior induced by salicylate depends on age and can be prevented by a single dose of 5-MeO-DMT(2020-01-08) Winne, Jessica; Boerner, Barbara C.; Malfatti, Thawann; Brisa, Elis; Doerl, Jhulimar; Nogueira, Ingrid; Leão, Emelie Katarina Svahn; Leão, Richardson NavesSalicylate intoxication is a cause of tinnitus and comorbidly associated with anxiety in humans. In a previous work, we showed that salicylate induces anxiety-like behavior and hippocampal type 2 theta oscillations (theta2) in mice. Here we investigate if the anxiogenic effect of salicylate is dependent on age and previous tinnitus experience. We also tested whether a single dose of DMT can prevent this effect. Using microwire electrode arrays, we recorded local field potential in young (4-5- month-old) and old (11-13-month-old) mice to study the electrophysiological effect of tinnitus in the ventral hippocampus (vHipp) and medial prefrontal cortex (mPFC) in an open field arena and elevated plus maze 1h after salicylate (300mg/kg) injection. We found that anxiety-like behavior and increase in theta2 oscillations (4-6 Hz), following salicylate pre-treatment, only occurs in young (normal hearing) mice. We also show that theta2 and slow gamma oscillations increase in the vHipp and mPFC in a complementary manner during anxiety tests in the presence of salicylate. Finally, we show that pre-treating mice with a single dose of the hallucinogenic 5-MeO-DMT prevents anxiety-like behavior and the increase in theta2 and slow gamma oscillations after salicylate injection in normal hearing young mice. This work further support the hypothesis that anxiety-like behavior after salicylate injection is triggered by tinnitus and require normal hearing. Moreover, our results show that hallucinogenic compounds can be effective in treating tinnitus-related anxiety.Artigo Salicylate induces anxiety-like behaviour and slow theta oscillation and abolishes the relationship between running speed and fast theta oscillation frequency(2018-08-28) Winne, Jessica; Franzon, Rafael; Miranda, Aron; Malfatti, Thawann; Patriota, João; Mikulovic, Sanja; Leão, Emelie Katarina Svahn; Leão, Richardson NavesSalicylate intoxication is a cause of tinnitus in humans and it is often used to produce tinnitus‐like perception in animal models. Here we assess whether salicylate induces anxiety‐like electrophysiological and behavioural signs. Using microwire electrode arrays, we recorded local field potential in the ventral and, in some experiments dorsal hippocampus, in an open field arena 1 hour after salicylate (300mg/kg) injection. We found that animals treated with salicylate moved dramatically less than saline treated animals. Salicylate‐treated animals showed a strong 4‐6Hz (type 2) oscillation in the ventral hippocampus (with smaller peaks in dorsal hippocampus electrodes). Coherence in the 4‐6Hz‐theta band was low in the ventral and dorsal hippocampus when compared to movement‐related theta coherence (7‐10Hz). Moreover, movement related theta oscillation frequency decreased and its dependency on running speed was abolished. Our results suggest that salicylate‐induced theta is mostly restricted to the ventral hippocampus. Slow theta has been classically associated to anxiety‐like behaviours. Here we show that salicylate application can consistently generate low frequency theta in the ventral hippocampus. Tinnitus and anxiety show strong comorbidity and the increase in ventral hippocampus low frequency theta could be part of this association.