Navegando por Autor "Maróstica Júnior, Mário Roberto"
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Artigo Metabolic dysfunctions promoted by AIN-93G standard diet compared with three obesity-inducing diets in C57BL/6J mice(Current Research in Physiology, 2022) Maia, Juliana Kelly da Silva; Aguiar, Lais Marinho; Moura, Carolina Soares de; Ballard, Cintia Reis; Roquetto, Aline Rissetti; Duarte, Gustavo H.B.; Costa, Larissa Bastos Eloy da; Torsoni, Adriana Souza; Farfan, Jaime Amaya; Maróstica Júnior, Mário Roberto; Cazarin, Cinthia Baú BetimResearchers from different fields have studied the causes of obesity and associated comorbidities, proposing ways to prevent and treat this condition by using a common animal model of obesity to create a profound energy imbalance in young adult rodents. However, to confirm the harmful effects of consuming a high-fat and hypercaloric diet, it is common to include normolipidic and normocaloric control groups in the experimental protocols. This study compared the effect of three experimental diets described in the literature – namely, a highfat diet, a high-fat and high-sucrose diet, and a high-fat and high-fructose diet – to induce obesity in C57BL/6 J mice with the standard AIN-93G diet as a control. We hypothesize that the AIN diet formulation is not a good control in this type of experiment because this diet promotes weight gain and metabolic dysfunctions similar to the hypercaloric diet. The metabolic data of animals fed the AIN-93G diet were similar to those of the highcalorie groups (development of steatosis and hyperlipidemia). However, it is important to emphasize that the group fed a high-fat diet had a higher percentage of total fat (p = 0.0002) and abdominal fat (p = 0.013) compared to the other groups. Also, the high-fat group responded poorly to glucose and insulin tolerance tests, showing a picture of insulin resistance. As expected, the intake of the AIN-93G diet promotes metabolic alterations in the animals like the high-fat formulations. Therefore, although this diet continues to be used as the gold standard for growth and maintenance, it warrants a reassessment of its composition to minimize the metabolic changes observed in this study, thus updating its fitness as a normocaloric model of a standard rodent dietArtigo Syzygium malaccense fruit supplementation protects mice brain against high-fat diet impairment and improves cognitive functions(Journal of Functional Foods, 2020-02) Maia, Juliana Kelly da Silva; Batista, Ângela Giovana; Mendonça, Monique Culturato Padilha; Soares, Edilene Siqueira; Dionísio, Ana Paula; Sartori, Cesar Renato; Höfling, Maria Alice da Cruz; Maróstica Júnior, Mário RobertoIn this study, we supplemented a high-fat diet (HF) with 5% powder of freeze-dried Syzygium malaccense fruit (HS) and investigated proofs of peripheral insulin resistance, hippocampal AKT–GSK3-β–tau activation, learning/memory performance, and brain frontal lobe oxidative stress. The intake of HS did not prevent weight gain but promoted peripheral insulin sensitivity, improved AKT signaling in the hippocampus, which prevented the activation of GSK3-β and lowered tau phosphorylation induced in HF. The results from the Morris water maze cognitive test were consistent with the lower tau-phosphorylation, once the HS group showed lower latency in the acquisition phase and more times crossing the target quadrant when compared with HF. The HS diet improved brain antioxidant enzyme activities. Overall, the intervention with S. malaccense fruit to mice was effective in minimizing cognitive deficit caused by HF consumption and in preventing risk markers associated with Alzheimer’s disease without reducing weight gain in mice