Navegando por Autor "Oliveira, Flavia de"

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Challenges of the intensive insulin therapy in experimental models of extensive burn injury
(World Journal Series, 2020-08-30) Quintana, Hananiah Tardivo; Baptista, Vivianne Izabelle de Araújo; Lazzarin, Mariana Cruz; Oliveira, Flavia de
Burn injuries (BI) above 40% of total body surface area (TBSA) are considered extensive and associated to systemic responses. The intensive insulin therapy (IIT) has been chosen as treatment because of its anabolic and anti-inflammatory properties, and by glycemic control. Several experimental models of extensive BI with IIT has just been studied, however they have many variables and challenges. Thus, this review aims to investigate the animal models of extensive BI with IIT, in order to better understanding benefits and limitations of this therapy. The review of papers published on the literature and indexed on the PubMed database was conducted by searching the keywords predetermined. Insulin administration after BI is able to revert hyperglycemia state, accelerate wound healing, decrease the mRNA expression of some pro-inflammatory cytokines, attenuate acute lung injuries, decrease inflammation in intestinal epithelium and attenuate the muscle loss. We can conclude, although there are limitations related to burn standard or insulin administration, the systemic benefits of ITT overcome limitations
Insulin modulates myogenesis and muscle atrophy resulting from skin scald burn in young male rats
(Elsevier, 2020-08-17) Quintana, Hananiah Tardivo; Baptista, Vivianne Izabelle de Araújo; Lazzarin, Mariana Cruz; Antunes, Hanna Karen Moreira; Le Sueur-Maluf, Luciana; Oliveira, Camila Aparecida Machado de; Oliveira, Flavia de
Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Materials and methods: Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C þ I), scald burn injury (SI), and SI with insulin (SI þ I). The SI groups were submitted to a 45% total body surface area burn, and the C þ I and SI þ I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Results: Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Conclusions: Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy
Presence of metalloproteinases 2 and 9 and 8-OHdG in the fibrotic process in skeletal muscle of Mdx mice
(Elsevier, 2020-01) Souza, Lidiane Begalli de; Maziero, Carla; Lazzarin, Mariana Cruz; Quintana, Hananiah Tardivo; Tomé, Tabata de Carvalho; Baptista, Vivianne Izabelle de Araújo; Oliveira, Flavia de
Inflammation and oxidative stress occurs in muscle of Duchenne muscular dystrophy (DMD). The relationship between a panel of biomarkers and the DMD outcome is necessary to indicate of disease progression and response to rehabilitation programs. The aim was to analyze the connective tissue of muscle of Mdx mice and immunoexpression of MMP-2, MMP-9, and 8-OHdG, which signalizes oxidative stress related to DNA damage. Biceps brachii of male C57BL/10 and C57BL/10-Dmdmdx mice was submitted to Hematoxylin-Eosin, Sirius red and immunohistochemistry (MMP-2, MMP-9 and 8-OHdG) analysis. Mdx showed focal lesions with intense inflammation and fibrosis related to immunoexpression of MMP-2 and MMP-9, proving the hypothesis that these MMPs are linked to muscular tissue degeneration, which can be regenerated by their inhibition, improving the treatment of DMD carriers. Histopathological findings related to centralized nuclei increase were related to higher 8-OHdG immunomarked nuclei in Mdx, which signalizes oxidative stress associated with DNA damage provoked by DMD. Such result shows that the evaluation of 8-OHdG during the evolution of the disease could be a method to evaluate DMD disease progression

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Navegando por Autor "Oliveira, Flavia de"