Navegando por Autor "Oliveira, Jéssica J."
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Artigo Dose-dependent effects of alcohol on seeking behavior and memory in the fish betta splendens(American Psychological Association, 2015) Luchiari, Ana Carolina; Chacon, Diana M.; Oliveira, Jéssica J.The present study tested the effects of alcohol on seeking behavior and memory in the Siamese fighting fish Betta splendens. We tested behavior using 5 alcohol concentrations: .00%, .10%, .25%, 1.00%, and 1.50% (vol/vol%). Drug seeking was tested using a conditioned place preference (CPP) paradigm, with a single 20-min exposure to alcohol. The effect of alcohol on memory was tested using a T-maze protocol with acute (20 min/day for 5 days) and chronic (20 min/day for 20 days) alcohol exposure and after alcohol withdrawal (20 min/day alcohol exposure for 15 days water exposure). In the CPP test, the higher acute alcohol doses (1.00 and 1.50%) induced seeking behavior, but the lower (.10%) and medium (.25%) doses did not. When the fish were tested after 37 days of alcohol exposure, the higher-dose groups still exhibited seeking behavior, indicating that these doses may have caused drug addiction. In the memory test, we observed a dose-dependent pattern with both the acute and chronic treatments. High alcohol doses (1.00 and 1.50%) impaired memory, and low alcohol doses (.10%) caused an anticipatory response. The withdrawal group did not exhibit differences in memory, suggesting some capacity for recovery. The low alcohol doses did not impair memory or cause drug seeking, whereas the high doses affected memory and caused prolonged seeking behavior. Therefore, a dual effect of alcohol was corroborated by our data, and Betta splendens may be an adequate animal model for high-throughput screening with alcoholArtigo Irish coffee: effects of alcohol and caffeine on object discrimination in zebrafish(Elsevier, 2016-02-02) Santos, Luana; Oliveira, Julia Ruiz; Oliveira, Jéssica J.; Silva, Priscila F.; Luchiari, Ana CarolinaMany studies regarding the effects of drugs investigate the acute and chronic use of alcohol, but only a few address the effects of caffeine and alcohol combined to the performance of the zebrafish in cognitive tasks. The zebrafish is an importantmodel for studying the effects of drugs on learning, because it has large genetic similarities to humans and the non-invasive administration of the substances favors translational bias of research. In this study, we observed the effects of alcohol and caffeine on zebrafish cognition through an object discrimination test. We noticed that animals subjected to acute alcohol dose and those under alcohol or caffeine withdrawal did not show discrimination. When fish were treated with associated alcohol and caffeine, those chronically treated with alcohol and subjected to moderate acute dose of caffeine showed learning of the task. Our results reinforce the harmful effects of the alcohol use on cognitive tasks, and suggest that continued use of high doses of caffeine cause cognitive impairment during withdrawal of the substance. However, the acute use of caffeine appears to reverse the harmful effects of alcohol withdrawal, allowing discriminative performance equivalent to control fish. Finally, we reiterate the use of zebrafish as a model for drug effects screening and search for active compounds that modulate the alcohol and caffeine effects