Navegando por Autor "Oliveira Júnior, Sergio Dantas de"

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Nutritional value, cellulase activity and prebiotic effect of melon residues (Cucumis melo L. reticulatus group) as a fermentative substrate
(Journal of Food and Nutrition Research, 2018) Santos, Everaldo Silvino dos; Rolim, Priscilla Moura; Oliveira Júnior, Sergio Dantas de; Oliveira, Ana Carmen dos Santos Mendes de; Macedo, Gorete Ribeiro de
Melon residues were used to produce cellulases (EC 3.2.1.4) and to investigate the prebiotic effect in vitro. Peel and seed flours were characterized regarding contents of dietetic fibre, lipids, total protein, cellulose, hemicelluloses and lignin. A kinetic study was carried out in bacteriological greenhouse during 24, 48, 72, 96, 120, 144 and 168 h. In melon seed flour, cellulose at 350 g·kg-1, hemicellulose at 79 g·kg-1 and lignin at 24 g·kg-1 were determined. Results showed, in terms of dietetic fibre and high protein contents, that both seeds and peels have good nutritional value. The highest content of lipids, 246 g·kg-1, was found in seeds, compared to only 36.3 g·kg-1 in the peel. The best activity for carboxymethyl cellulase in 144 h of fermentation was 1.045 U·g-1, while the activity with filter paper after 96 h of cultivation was 0.190 U·g-1. Melon seed flour demonstrated to be a good substrate for the growth of bifidobacteria with 8h for fermentation, and it was tolerant to the action of bile salts at 8 h of fermentation. Melon residues can be taken as a potential prebiotic ingredient and a source of cellulolytic enzymes
Pretreatments of Carnauba (Copernicia prunifera) straw residue for production of cellulolytic enzymes by Trichorderma reesei CCT-2768 by solid state fermentation
(Elsevier, 2018-02) Santos, Everaldo Silvino dos; Silva, Francinaldo Leite da; Campos, Alan de Oliveira; Santos, Davi Alves dos; Oliveira Júnior, Sergio Dantas de; Padilha, Carlos Eduardo de Araújo; Sousa Junior, Francisco Canindé de; Macedo, Gorete Ribeiro de
In this study the effect of pretreatments, such as hydrothermal (HT) with hydrogen peroxide-alkaline (HP-A), acid-alkaline (AA), or alkaline (AL) pretreatments, on the Carnauba (Copernicia prunifera) straw residueda plant native to Brazil that is used to produce wax das well as the use of the pretreated biomass for the production of lignocellulolytic enzymes (cellulases and xylanases) by Trichoderma reesei CCT2768 using solid state fermentation (SSF) were evaluated. The untreated and pretreated biomasses were characterized by using the Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy assays. A kinetic study was carried out to estimate the best time for producing cellulases (FPase and CMCase) and xylanases. HP-A pretreatment was the only one that simultaneously reduced hemicellulose (removal of 60.72%), lignin (removal of 50.71%) and pretreatment yield of 59.28%. FPase (0.9 U/g) and CMCase (13 U/g) production in the case of this pretreatment was optimum, while AL pretreatment was ideal for xylanase (99.5 U/g). The use of Carnauba (Copernicia prunifera) straw residue coupled with HP-A pretreatment and SSF shows promise for the production of lignocellulolytic enzymes

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Navegando por Autor "Oliveira Júnior, Sergio Dantas de"