Navegando por Autor "Peixoto, Rephany Fonseca"
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Artigo Dual immune effect of iNKT cells considering human cutaneous and visceral leishmaniasis: an example of cell plasticity according to different disease scenarios(Scandinavian Journal of Immunology, 2018-05) Maciel, Bruna Leal Lima; Macêdo, Bruna de Góis; Peixoto, Rephany Fonseca; Gomes, Julliana de Assis Silva; Azevedo, Fátima de Lourdes Assunção Araújo de; Veras, Robson Cavalcanti; Medeiros, Isac Almeida de; Grisi, Teresa Cristina Soares de Lima; Araújo, Demétrius Antônio Machado de; Amaral, Ian Porto Gurgel do; Lima, Tatjana Keesen de SouzaAlthough the semi-invariant natural killer T cells (iNKT) are a small subpopulation of cells in the peripheral blood, they are presumed to play a role in early stages of infection against various pathogens, including protozoa. This work investigates the activation status and cytokine profile of iNKT cells during human Leishmania infantum and Leishmania braziliensis infection. We studied iNKT cells in patients with symptomatic active visceral leishmaniasis (AVL) (n = 8), patients with symptomatic active cutaneous leishmaniasis (ACL) (n = 13), negative endemic controls (NEC) (n = 6) and non-endemic controls (NonEC) (n = 6), with and without total Leishmania antigen stimulus (TLA). The number of iNKT cells in the peripheral blood of patients with ACL and AVL unaltered in relation to control groups. Moreover, the iNKT cells from ACL showed a hyperactivation profile compared to patients with AVL. Additionally, TLA induced IFN-gamma production in iNKT cells from patients with ACL, while in iNKT of patients with AVL, TLA induced a decrease in this cytokine. Higher IL-17 and IL-10 production by iNKT cells from patients with ACL were also observed compared to all other groups. There were no changes in iNKT IL-10-producing cells in AVL after TLA stimulation. However, TLA induced increase in IL-10 in iNKT cells in patients with ACL. These findings suggest that, although iNKT cells showed distinct profiles in patients with ACL and AVL, they play a dual role in immune modulation in both Leishmania infectionsArtigo Molecular and clinical epidemiological surveillance of dengue virus in Paraíba, northeast Brazil(Revista da Sociedade Brasileira de Medicina Tropical, 2017-02) Maciel, Bruna Leal Lima; Guerra-Gomes, Isabel Cristina; Peixoto, Rephany Fonseca; Oliveira, Camila Alves; Sarmento, Maria Izabel Ferreira; Pachá, Anna Stella Cysneiros; Araújo, Josélio Maria Galvão; Amaral, Ian Porto Gurgel; Keesen, Tatjana Souza LimaDespite being the most prevalent arboviral disease worldwide, dengue has been neglected lately. However, recent epidemics of arboviruses such as Zika and chikungunya in locations throughout the world have alerted health authorities to these diseases. This study evaluated the incidence pattern of dengue, its clinical characteristics, and co-circulation of serotypes from 2007 to 2015 in Paraiba State, Northeast Brazil. Methods: Data on dengue cases from 2007 to 2015 were extracted from clinical reports of the National System for Notifiable Diseases [Sistema Nacional de Agravos de Notificação (SINAN)] of Brazil provided by the Paraiba Health Department. Reverse transcription polymerase chain reaction (RT-PCR) assays for dengue serotypes were carried out on plasma samples obtained from patients with suspected dengue. The data were analysed using descriptive statistics.Results: According to clinical features, dengue fever [n = 39,083 (70.2%)] and dengue without warning signs [n = 15,365 (27.7%)] were the most common classifications of dengue. On RT-PCR, DENV 1 was the most commonly identified serotype (80.5%) in all years studied. Co-circulation of all four DENV serotypes was observed in 2013 and 2014. Furthermore, we observed an increase in dengue notifications in 2015, possibly due to the rise of Zika and chikungunya. Conclusions: Our findings support the hypothesis that co-circulation of the four DENV serotypes may be a reason for the increased prevalence of severe forms of dengue in the years studied. This study may contribute to directing research, health policy, and financial resources toward reducing poorly controlled epidemic diseases