Navegando por Autor "Santos, José R."
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Artigo Molecular, Neurochemical, and Behavioral Hallmarks of Reserpine as a Model for Parkinson’s Disease: New Perspectives to a Long-Standing Model(Wiley, 2015-03-02) Leão, Anderson H.F.F.; Sarmento-Silva, Aldair J.; Santos, José R.; Ribeiro, Alessandra M.; Silva, Regina H.Artigo Non-visual exploration of novel objects increases the levels of plasticity factors in the rat primary visual cortex(2018-10-16) Pereira, Catia M.; Freire, Marco A.M.; Santos, José R.; Guimarães, Joanilson S.; Dias-Florencio, Gabriella; Santos, Sharlene; Pereira, Antonio; Ribeiro, Sidarta Tollendal GomesBackground. Historically, the primary sensory areas of the cerebral cortex have been exclusively associated with the processing of a single sensory modality. Yet, the presence of tactile responses in the primary visual (V1) cortex has challenged this view, leading to the notion that primary sensory areas engage in cross-modal processing, and that the associated circuitry is modifiable by such activity. To explore this notion, here we assessed whether the exploration of novel objects in the dark induces the activation of plasticity markers in the V1 cortex of rats. Methods. Adult rats were allowed to freely explore for 20 min a completely dark box with four novel objects of different shapes and textures. Animals were euthanized either 1 (nD5) or 3 h (nD5) after exploration. A control group (nD5) was placed for 20 min in the same environment, but without the objects. Frontal sections of the brains were submitted to immunohistochemistry to measure protein levels of egr-1 and c-fos, and phosphorylated calcium-dependent kinase (pCaKMII), in V1 cortex. Results. The amount of neurons labeled with monoclonal antibodies against c-fos, egr-1 or pCaKMII increased significantly in V1 cortex after one hour of exploration in the dark. Three hours after exploration, the number of labeled neurons decreased to basal levels. Conclusions. Our results suggest that non-visual exploration induces the activation of immediate-early genes in V1 cortex, which is suggestive of cross-modal processing in this area. Besides, the increase in the number of neurons labeled with pCaKMII may signal a condition promoting synaptic plasticity.Artigo Variants in SNCA gene are associated with parkinson’s disease risk and cognitive symptoms in a brazilian sample(Frontiers in Aging Neuroscience, 2017-06-20) Godeiro Junior, Clecio de Oliveira; Campêlo, Clarissa Loureiro das Chagas; Cagni, Fernanda Carvalho; Figueredo, Diego de Siqueira; Oliveira Junior, Luiz Gonzaga; Silva Neto, Antônio Braz; Macêdo, Priscila T.; Santos, José R.; Izídio, Geison Souza; Ribeiro, Alessandra Mussi; Andrade, Tiago Gomes de; Silva, Regina Helena; 0000-0002-4312-1633Genetic susceptibility contributes to the etiology of sporadic Parkinson’s Disease (PD) and worldwide studies have found positive associations of polymorphisms in the alphasynuclein gene (SNCA) with the risk for PD. However, little is known about the influence of variants of SNCA in individual traits or phenotypical aspects of PD. Further, there is a lack of studies with Latin-American samples. We evaluated the association between SNCA single nucleotide polymorphisms (single nucleotide polymorphisms, SNPs –rs2583988, rs356219, rs2736990, and rs11931074) and PD risk in a Brazilians sample. In addition, we investigated their potential interactions with environmental factors and specific clinical outcomes (motor and cognitive impairments, depression, and anxiety). A total of 105 PD patients and 101 controls participated in the study. Single locus analysis showed that the risk allele of all SNPs were more frequent in PD patients (p < 0.05), and the associations of SNPs rs2583988, rs356219, and rs2736990 with increased PD risk were confirmed. Further, the G-rs356219 and C-rs2736990 alleles were associated with early onset PD. T-rs2583988, G-rs356219 and C-2736990 alleles were significantly more frequent in PD patients with cognitive impairments than controls in this condition. In addition, in a logistic regression model, we found an association of cognitive impairment with PD, and the practice of cognitive activity and smoking habits had a protective effect. This study shows for the first time an association of SNCA polymorphism and PD in a South-American sample. In addition, we found an interaction between SNP rs356219 and a specific clinical outcome, i.e., the increased risk for cognitive impairment in PD patients.