Navegando por Autor "Silva, Iago Bruno França Pereira da"
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TCC Obtaining and characterization of a nanosystem associated with the stiga16 peptide derived from the venom of the scorpion Tityus stigmurus(Universidade Federal do Rio Grande do Norte, 2025-01-09) Silva, Iago Bruno França Pereira da; Ferreira, Sarah de Sousa; Costa, Rita de Kassia Oliveira da; Silva, Alessandra Daniele da; Fernandes-Pedrosa, Matheus de Freitas; Pedrosa, Matheus de Freitas Fernandes; Silva, Alessandra Daniele da; https://orcid.org/0000-0003-1650-3836; http://lattes.cnpq.br/1245062709759151; https://orcid.org/0000-0003-4221-9580; http://lattes.cnpq.br/2929963416385218; 0009-0007-4504-6888; https://lattes.cnpq.br/3343322391203406; Rêgo, Manoela Torres do; https://orcid.org/0000-0002-8535-1533; http://lattes.cnpq.br/0735782214033188; Brito, Carlos Ramon do Nascimento; https://orcid.org/0000-0003-4882-5215; http://lattes.cnpq.br/0574891483598906The growing problem of microbial resistance, which is developing rapidly worldwide, drives the need to search for molecules of natural origin with antibacterial action. StigA16, an antimicrobial peptide analog of Stigmurin present in the venom of the scorpion Tityus stigmurus, has shown a broad spectrum of antimicrobial action with superior action to the native peptide. In this study, aiming to reduce toxicity, increase antibacterial activity and decrease enzymatic degradation, a nanosystem was developed and characterized using chitosan nanoparticles with the StigA16 peptide incorporated. In addition, the in vitro antibacterial action of the nanosystem associated with the peptide was evaluated. The chitosan nanoparticles were obtained through the ionotropic gelation technique by the incorporation method, with the peptide associated with the crosslinking agent tripolyphosphate (TPP+A16) or chitosan (NPQ+A16). The physicochemical characteristics of the nanosystem (particle size, zeta potential and polydispersity index) were evaluated by the dynamic light scattering method and the incorporation efficiency was determined by the bicinchoninic acid method. In addition, the antibacterial action against the Staphylococcus aureus strain (ATCC 25923) was evaluated in vitro by the broth microdilution method. TPP+A16 and NPQ+A16 revealed a similar particle size, with approximately 135.6 nm and 128.6 nm, zeta potential +21.0 and +21.9, respectively, and polydispersity index between 0.4 and 0.5. The systems presented physicochemical stability after 40 days of obtaining the formulation and high incorporation of the bioactive molecule (above 98%), presenting an increasing release profile represented by the Higuchi model. TPP+A16 and NPQ+A16 showed antimicrobial action against S. aureus, at non-hemolytic concentrations. Thus, the association of the chitosan nanosystem with StigA16 consists of a promising approach for the potential therapeutic application of this peptide as a future antibiotic agent