Navegando por Autor "Silva, Nayane Macedo Portela da"

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Recovery and puriﬁcation of chitosanaseproduced by Bacillus cereus using expandedbed adsorption and central composite design
(Wiley, 2016-02) Santos, Everaldo Silvino dos; Macedo, Gorete Ribeiro de; Araújo, Nathália Kelly de; Pimentel, Vanessa Carvalho; Silva, Nayane Macedo Portela da; Padilha, Carlos Eduardo de Araújo
This study presents a system for expanded bed adsorption for the puriﬁcation of chitosanasefrom broth extract in a single step. A chitosanase-producing strain was isolated and identiﬁedas Bacillus cereus C-01 and used to produce chitosanases. The expanded bed adsorptionconditions for chitosanase puriﬁcation were optimized statistically using STREAMLINETMDEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were deﬁned by thequality criteria puriﬁcation factor (P) and enzyme yield to optimize the chromatographicprocess. Statistical analyses showed that the optimum conditions for the maximum P were150 cm/h load ﬂow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height.Distributor height had a strong inﬂuence on the process, considerably affecting both theP and enzyme yield. Optimizing the puriﬁcation variables resulted in an approximately3.66-fold increase in the P compared with the value under nonoptimized conditions. Thissystem is promising for the recovery of chitosanase from B. cereus C-01 and is economicallyviable because it promotes the reduction steps

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