Navegando por Autor "Viana, Rony Lucas Silva"
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Artigo Antioxidant stability enhancement of carotenoid rich-extract from Cantaloupe melon (Cucumis melo L.) nanoencapsulated in gelatin under different storage conditions(Elsevier, 2021) Morais, Ana Heloneida de Araújo; Oliveira, Grazielle Louise Ribeiro de; Medeiros, Isaiane; Nascimento, Sara Sayonara da Cruz; Viana, Rony Lucas Silva; Porto, Dayanne Lopes; Rocha, Hugo Alexandre Oliveira; Aragão, Cícero Flávio Soares; Maciel, Bruna Leal Lima; Assis, Cristiane Fernandes de; Passos, Thais Souza; https://orcid.org/0000-0002-6460-911XThe study evaluated the potential and antioxidant stability of nanoencapsulated carotenoid-rich extract (CE) from Cantaloupe melon (EPG). DPPH and ABTS radical scavenging assays were used to investigate the nano encapsulation effect on antioxidant potential. CE and EPG stability were evaluated at 25 ◦C and 5 ◦C, with and without light (1600 lx) for 60 days, determining the β-carotene concentration by UHPLC and antioxidant po tential by ABTS. The antioxidant potential of carotenoids increased after nanoencapsulation (57–59%). After 60 days, there was low retention of β-carotene (0–43.6%) in the CE, mainly at 25 ◦C light (0.00%) and dark (10.0%), and total loss of activity in the four conditions. EPG preserved the β-carotene concentration in the dark at 25 ◦C (99.0%) and in the light (83.1%) and dark (99.0%) at 5 ◦C, maintaining the antioxidant potential (68.7–48.3%). Therefore, EPG enhanced and stabilized the antioxidant potential of carotenoids, beneficial to human health.Artigo Commercial fucoidans from Fucus vesiculosus can be grouped into antiadipogenic and adipogenic agents(MDPI, 2018-06-04) Oliveira, Ruth Medeiros; Câmara, Rafael Barros Gomes da; Monte, Jessyka Fernanda Santiago; Viana, Rony Lucas Silva; Melo, Karoline Rachel Teodosio; Queiroz, Moacir Fernandes; Filgueira, Luciana Guimarães Alves; Oyama, Lila Missae; Rocha, Hugo Alexandre de OliveiraFucus vesiculosus is a brown seaweed used in the treatment of obesity. This seaweed synthesizes various bioactive molecules, one of them being a sulfated polysaccharide known as fucoidan (FF). This polymer can easily be found commercially, and has antiadipogenic and lipolytic activity. Using differential precipitation with acetone, we obtained four fucoidan-rich fractions (F0.5/F0.9/F1.1/F2.0) from FF. These fractions contain different proportions of fucose:glucuronic acid:galactose:xylose:sulfate, and also showed different electrophoretic mobility and antioxidant activity. Using 3T3-L1 adipocytes, we found that all samples had lipolytic action, especially F2.0, which tripled the amount of glycerol in the cellular medium. Moreover, we observed that FF, F1.0, and F2.0 have antiadipogenic activity, as they inhibited the oil red staining by cells at 40%, 40%, and 50%, respectively. In addition, they decreased the expression of key proteins of adipogenic differentiation (C/EBPα, C/EBPβ, and PPARγ). However, F0.5 and F0.9 stimulated the oil red staining at 80% and increased the expression of these proteins. Therefore, these fucoidan fractions have an adipogenic effect. Overall, the data show that F2.0 has great potential to be used as an agent against obesity as it displays better antioxidant, lipolytic and antiadipogenic activities than the other fucoidan fractions that we testedArtigo The protective role of sulfated polysaccharides from green seaweed Udotea flabellum in cells exposed to oxidative damage(MDPI, 2018-04-20) Presa, Fernando Bastos; Marques, Maxsuell Lucas Mendes; Viana, Rony Lucas Silva; Nobre, Leonardo Thiago Duarte Barreto; Costa, Leandro Silva; Rocha, Hugo Alexandre de OliveiraSeaweed is a rich source of bioactive sulfated polysaccharides. We obtained six sulfated polysaccharide-rich fractions (UF-0.3, UF-0.5, UF-0.6, UF-0.7, UF-1.0, and UF-2.0) from the green seaweed Udotea flabellum (UF) by proteolytic digestion followed by sequential acetone precipitation. Biochemical analysis of these fractions showed that they were enriched with sulfated galactans. The viability and proliferative capacity of 3T3 fibroblasts exposed to FeSO4 (2 µM), CuSO4 (1 µM) or ascorbate (2 mM) was not affected. However, these cells were exposed to oxidative stress in the presence of FeSO4 or CuSO4 and ascorbate, which caused the activation of caspase-3 and caspase-9, resulting in apoptosis of the cells. We also observed increased lipid peroxidation, evaluated by the detection of malondialdehyde and decreased glutathione and superoxide dismutase levels. Treating the cells with the ultrafiltrate fractions (UF) fractions protected the cells from the oxidative damage caused by the two salts and ascorbate. The most effective protection against the oxidative damage caused by iron was provided by UF-0.7 (1.0 mg/mL); on treatment with UF-0.7, cell viability was 55%. In the case of copper, cell viability on treatment with UF-0.7 was ~80%, but the most effective fraction in this model was UF-2.0, with cell viability of more than 90%. The fractions, mainly UF-0.7 and UF-2.0, showed low iron chelating activity, but high copper chelating activity and total antioxidant capacity (TAC). These results suggested that some of their protective mechanisms stem from these properties