CB - DBF - Artigos publicados em periódicos
URI Permanente para esta coleçãohttps://repositorio.ufrn.br/handle/1/104
Navegar
Navegando CB - DBF - Artigos publicados em periódicos por Assunto "Animal model"
Agora exibindo 1 - 2 de 2
- Resultados por página
- Opções de Ordenação
Artigo Lithium and valproate prevent methylphenidate-induced mania-like behaviors in the hole board test(Elsevier, 2016-08-26) Souza, L.S.; Soares, Bruno Lobão; Silva, E.F.; Santos, W.B.; Asth, L.; Soares-Rachetti, V.P.; Medeiros, I.U.; Gavioli, E.C.Manic bipolar is diagnosed by psychomotor agitation, increased goal-directed activity, insomnia, grandiosity, excessive speech, and risky behavior. Animal studies aimed to modeling mania are commonly based in psychostimulants-induced hyperlocomotion. The exploration of other behaviors related with mania is mandatory to investigate this phase of bipolar disorder in animals. In this study, the hole board apparatus was suggested for evaluating mania-like behaviors induced by the psychostimulant methylphenidate. The treatment with methylphenidate (10 mg/kg, ip) increased locomotion in the open field test. The pretreatment with lithium (50 mg/kg, ip) and valproate (400 mg/kg, ip) significantly prevented the hyperlocomotion. In the hole-board test, methylphenidate increased interactions with the central and peripheral holes and the exploration of central areas. Lithium was more effective than valproate in preventing all the behavioral manifestations induced by the psychostimulant. These findings were discussed based on the ability of methylphenidate-treated mice mimicking two symptoms of mania in the hole board test: goal-directed action and risk-taking behavior. In conclusion, the results point to a new approach to study mania through the hole board apparatus. The hole board test appears to be a sensitive assay to detect the efficacy of antimanic drugs.Artigo The elevated T-maze task as an animal model to simultaneously investigate the effects of drugs on long-term memory and anxiety in mice(Elsevier, 2012-04-10) Soares, Bruno Lobão; Asth, Laila; André, Eunice; Soares, Vanessa de Paula; Gavioli, Elaine CristinaThe elevated T-maze (ETM) is an apparatus derived from the elevated plus-maze test, which is used to evaluate anxiety. Because anxiety is a biasing factor in models of memory, this study proposed the ETM as a task for the simultaneous assessment of memory and anxiety in mice. The ETM consists of one enclosed and two open arms. The procedure is based on the avoidance of open spaces learned during training session, in which mice were exposed to the enclosed arm as many times as needed to stay 300 s. In the test session, memory is assessed by re-exposing the mouse to the enclosed arm and the latency to enter an open arm was recorded. The anxiolytic diazepam (DZP; 1 or 2 mg/kg) and the amnestic biperiden (BPR; 0.5, 1 or 3 mg/kg) were injected at three distinct times: pre-training, post-training, and pre-test. Pretraining administration of BPR 1 and DZP 2 increased the number of trials needed to reach the avoidance criterion, suggesting a passive avoidance learning impairment. However, BPR induced hyperlocomotion, which could bias the interpretation of any BPR-induced effects during the training session. Pre-training injection of BPR did not affect the spontaneous increase in the latency to enter an open arm between trials, while DZP reduced latencies in the first three trials suggesting anxiolysis. In the test session, pre-training injection of BPR 1 and DZP 2 reduced latencies to enter an open arm, indicating memory impairment. Post-training and pre-test injection of DZP or BPR did not affect memory. In conclusion, the proposed ETM task is practical for the detection of the anxiolytic and amnesic effects of drugs.