Programa de Pós-Graduação em Ciências Farmacêuticas
Permanent URI for this communityhttps://repositorio.ufrn.br/handle/123456789/11920
Browse
2 results
Search Results
Doctoral Thesis Resíduo agroindustrial de Agave sisalana como um inovador insumo cosmético antioxidante: avaliação in vitro e in vivo(Universidade Federal do Rio Grande do Norte, 2022-12-20) Barreto, Stella Maria Andrade Gomes; Ferrari, Márcio; Giordani, Raquel Brandt; http://lattes.cnpq.br/5032750980466610; https://orcid.org/0000-0002-2425-7586; http://lattes.cnpq.br/5782539548696465; https://orcid.org/0000-0001-7830-626X; http://lattes.cnpq.br/2234955265649411; Lima, Adley Antonini Neves de; https://orcid.org/0000-0002-3798-3915; http://lattes.cnpq.br/4061809277935577; Damasceno, Gabriel Azevedo de Brito; Tavares, Josean Fechine; Isaac, Vera Lucia BorgesAgave sisalana (sisal) is a plant extensively cultivated in the northeast region to obtain a hard fiber used to produce string and rope. However, only a small portion of the gross weight of sisal leaves is used, and the rest is discarded as residue. This residue has metabolites with potential antioxidant activity in its chemical constitution. In view of this, the objective of this work was, from the residue of A. sisalana, to obtain and characterize a hydroethanolic and a purified absorption, to evaluate the antioxidant potential in vitro and in vivo and to develop a nanoemulsion for carrier fractions to be applied as a cosmetic product antioxidant. From the residue, a recipe rich in polysaccharides (FRE) were received and, from the FRE, another recipe was through ultrafiltration dialysis (FREP). The qualitative composition was evaluated using liquid chromatography coupled to mass spectrometry (LC-MS). Both fractions were evaluated for their antioxidant activity in vitro, using different spectrophotometric techniques, culture medium and in vivo, using the Caenorhabditis elegans model. Furthermore, an experimental factorial design (2³+3PC) was designed to obtain a nanoemulsified system and its stability evaluated. FRE and FREP are mainly composed of terpenoids and O-glycosylated metabolites. FRE showed greater ability to directly inhibit DPPH and ABTS radicals, while FREP showed greater metal chelating activity (Cu2+ and Fe2+). The two fractions promote a protective effect against oxidative stress in 3T3 cells. In C. elegans models, FRE and FREP promoted intracellular reduction of ROS and increased survival representation. The expression of gst-4:GFP was positive for FRE whereas, for FREP, a reduction in expression was observed. The nanoemulsion consisting of 20% oil phase, 5% surfactants and 75% aqueous phase was considered stable for 90 days at different temperatures. The use of sisal residue as a new antioxidant input for the cosmetic industry can bring environmental benefits and contribute to the socioeconomic development of the region.Master Thesis Utilização do subproduto do beneficiamento do sisal (Agave sisalana Perrine): desenvolvimento de nanoemulsões cosméticas e avaliação da segurança e eficácia(2017-03-31) Barreto, Stella Maria Andrade Gomes; Ferrari, Márcio; Giordani, Raquel Brandt; ; http://lattes.cnpq.br/5032750980466610; ; http://lattes.cnpq.br/5782539548696465; ; http://lattes.cnpq.br/2234955265649411; Rocha, Hugo Alexandre de Oliveira; ; http://lattes.cnpq.br/4651814546820796; Isaac, Vera Lúcia Borges; ; http://lattes.cnpq.br/4842462513285606The processing of the leaves of Agave sisalana provides fiber, with added value for the sisal industry, and the rest of the plant material, known as by-product, is thrown and generates environmental impact. Different applications have been attributed, however, there are no scientific researches that aim to use this product as a cosmetic raw material. The objective of this research was to obtain and characterize polysaccharides-enriched fraction (FrE) from the Agave sisalana by-product of, assess the safety in vitro and in vivo, as well as to evaluate the antioxidant activity in vitro, and then to develop a nanoemulsion with moisturizer effect. From the liquid portion of the by-product obtained the crude extract (EB) and two fractions resulting from extractive process. The EB and the fractions were evaluated for the presence of sugars, proteins and polyphenols. Safety was evaluated by cytotoxicity and phototoxicity in vitro assays using the colorimetric method of Neutral Red. Irritability was evaluated by the primary contact test (patch test). The antioxidant capacity was assessed using the DPPH scavenging tests and molybdenum method. Different systems were obtained by means of pseduodiagrama ternary containing, or not, 0.5% FrE. The selected formulations were submitted to preliminary stability tests and accelerated in different temperatures conditions for a period of 90 days. The moisturizing efficacy was evaluated by water content of the stratum corneum and transepidermal water loss for a period of 5 hours. The samples presented as chemical constituents, sugars, polyphenols and proteins. The FrE showed no phototoxic potential or cytotoxic, and was not irritating to skin of volunteers and can be considered an asset with antioxidant property Nanoemulsion containing 40% oil phase, 50% aqueous phase and 10% surfactants was stable for 90 days.The nanoemulsion was able to significantly increase the water content and maintain skin barrier function after 5 hours from a single application. The fraction obtained from the industrial by-product of Agave sisalana demonstrated a promising profile as raw material for cosmetic skin care with moisturizing and antioxidant capacity, together with the possibility of adding value to industrial waste, reducing the damage caused to the environment by disposing of this material.