Programa de Pós-Graduação em Ciências Farmacêuticas
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Master Thesis Avaliação da estabilidade da associação de isoniazida e rifampicina(Universidade Federal do Rio Grande do Norte, 2010) Barbosa, Manuela Bernardo Câmara; Raffin, Fernanda Nervo; Oliveira, Eduardo de Jesus; http://lattes.cnpq.br/1293090609428232; Aragão, Cícero Flávio Soares; http://lattes.cnpq.br/9657118649043311; http://lattes.cnpq.br/1052562183676637; http://lattes.cnpq.br/7501998432159911; Moura, Túlio FIávio Accioly de Lima eTuberculosis is a disease of older, known in the worid. The main treatment for this disease is achieved with the fixed-dose combination formulations, involving the main drugs. This study aims to determine the quality and conduct the first evaluation of stability of capsules of isoniazid and rifampicin formulations nationals with respect to physical, Chemical properties and dissolution after storage in accelerated conditions (40 ° C temperature and 75% RH) and under conditions of long duration (30 ° C temperature and 75% relative humidity). USP methods were used previously validated, based on visible spectrophotometry for the dissolution of rifampicin and high performance liquid chromatography for the content of rifampicin and isoniazid and disbandment. Four samples were submitted to six months of accelerated stability study and 12 months of stability study of long duration. The drugs were subjected to stressful conditions, performing hydrolysis at different pH values and temperatures. All formulations tested showed satisfactory for pharmacopoeial testing. In degradation studies, there were products resulting from the conditions imposed on drugs. But a more careful analysis, using methods more sensitive and specific instruments is needed to separate and quantify these degradation products. The formulations tested are nationals of good quality and monitoring the appearance of degradation products is needed throughout the stability study.Master Thesis Avaliação da estabilidade de formulações líquidas orais de sulfadiazina obtidas a partir de comprimidos(2019-03-20) Costa, Brunna Soares Rodrigues; Gomes, Ana Paula Barreto; Verissimo, Lourena Mafra; ; ; ; Azevedo, Eduardo Pereira de; ; Nogueira, Fernando Henrique Andrade;Sulfadiazine (SDZ) is one of the drugs used for the treatment of congenital toxoplasmosis, however the only commercially available oral formulation is the tablet (500 mg). One of the strategies to solve this problem is through the transformation of pharmaceutical forms. Thus, the present work aimed to prepare and evaluate the physical, chemical and microbiological stability of extemporaneous suspensions of SDZ (100 mg / mL) using tablets (FURP-Sulfadiazine). The suspension vehicles used were: A - 85% simple syrup and 1% (w/v) methylcellulose; and B - sorbitol 70%. Stability was evaluated during days 0, 7, 14 and 30 under storage at controlled temperatures of 5 °C ± 3 °C and 22.5 °C ± 2.5 °C. The physical characteristics analyzed were the organoleptic characteristics, the pH, the particle size and the viscosity. The concentration of SDZ was determined by a method developed and validated using ultra-high performance liquid chromatography (CLUE). Microbiological attributes of non-sterile pharmaceutical forms were also evaluated by adding p-aminobenzoic acid to the culture media in order to neutralize the antimicrobial activity of SDZ. pH and viscosity showed significant differences on study days. The particle size distribution was constant up to 14 days of study for both formulations. However, only the SDZ A suspension presented reliable results within the pharmacopoeial limits, probably influenced by the solubility of the active substance at different pH, particle sedimentation and viscosity reduction. In addition, during the study no microbial contamination was observed. Based on the results, it can be concluded that SDZ A suspension at room temperature is stable for 14 days and is a viable alternative for the pediatric treatment of congenital toxoplasmosis.Master Thesis Caracterização e desenvolvimento de metodologias para análise simultânea de ésteres derivados do ácido p-cumárico por métodos instrumentais(2017-06-29) Fidelis, Maxciara Agda Vicente Pereira; Aragão, Cicero Flávio Soares; Nogueira, Fernando Henrique Andrade; ; ; ; Gomes, Ana Paula Barreto; ; Ferreira, Leandro de Santis; ; Conceição, Marta Maria da;The development of new drugs is an activity of great importance for the pharmaceutical industry and involves several steps, among them the quality and safety monitoring of the substances of interest. P-coumaric acid, via an esterification process, gives methyl p-coumarate, ethyl p-coumarate and isopropyl p-coumarate, which are targets of several biological studies, but information about its physicochemical characteristics, stability and analytical methodology for its identification and quantification are scarce in the literature. In this context, this work aims at the thermal and chromatographic evaluation of these esters, aiming to evaluate their stability and physicochemical characteristics contributing in the new drugs research and quality control of drugs that will present these molecules as active principle. The analysis by DSC, DTA and TG showed that the p-coumaric acid derivatives, despite their structural similarity, have different thermal behavior, so that it is possible to use these techniques to identify them. Methyl p-coumarate and ethyl p-coumarate showed melting point at 131 ° C and 65 ° C, respectively, since the p-coumarate isopropyl did not show a melting process. The three samples have good thermal stability, with methyl p-coumarate being the most stable. A method for simultaneous identification of these compounds by UHPLC was developed, the method was also efficient in the identification of its degradation products when applied in the study of forced degradation.Master Thesis Desenvolvimento de emulsões contendo extrato de Kalanchoe brasiliensis Cambess e avaliação clínica da eficácia hidratante(2016-01-26) Rodrigues, Rayllan de Oliveira; Ferrari, Marcio; ; ; Gomes, Ana Paula Barreto; ; Rocha Filho, Pedro Alves da;Kalanchoe brasiliensis Cambess specie, belonging to the Crassulaceae family, presents flavonoids, polysaccharides and ascorbic acid in its chemical composition. These compounds are generally used in cosmetics formulations as antioxidants, moisturizers and anti-aging properties. This research aimed to development moisturizing emulsions add or not with hydroethanolic extract of the Kalanchoe brasiliensis leaves and evaluated its moisturizing clinical efficacy. Formulations have been developed with different concentrations of Sodium Acrylates/ Beheneth-25 Methacrylate Crosspolymer (and) Hydrogenated Polydecene (and) Lauryl Glucoside (Novemer™EC-2 polymer), incorporated to Acrylates/C10-30 Alkyl Acrylate Crosspolymer (Carbopol® Ultrez-20). The formulations were added or not with plant extract 0.5% (w/w) and subjected to accelerated stability tests. In accelerated stability study, the samples were stored at different temperatures for 90 days. The organoleptic characteristics, pH and rheological behavior were evaluated. The formulation containing 3.0% Novemer™EC-2 and 0.4% Carbopol® Ultrez-20 added with hydroethanolic extract of K. brasiliensis 0.5% was considered stable. Moisturizing clinical efficacy was evaluated by capacitance and transepidermal water loss methodologies. The formulation containing extract compared to vehicle promoted increase significantly statistical of the water content of the stractum corneum and decrease of transepidermal water loss, promoting barrier effect until five hours after single application of the formulation. The emulsion developed with 0.5% of hydroethanolic extract of K. brasiliensis leaves presented moisturizing efficacy acting possibly by occlusion and humectation mechanisms, demonstrating potential use as a moisturizing cosmetic product.Master Thesis Estudo de pré-formulação para a obtenção de uma formulação de captopril para uso pediátrico(2013-08-29) Goes, Janaina da Silva; Raffin, Fernanda Nervo; Aragão, Cicero Flávio Soares; ; ; ; Morais, Waldenice de Alencar; ; Fonteles, Marta Maria de França;Nowadays, drugs used in children are adapted from solid dosage forms developed for adults. Captopril in solid dosage form is widely adapted in hospitals into a liquid formulation. Its stability in aqueous solutions is reduced because it undergoes oxidation, forming captopril disulfide. In order to ensure a stable and accurate dosage form, a pre-formulation study was developed for obtaining a stable formulation of a powder for preparation of a captopril solution for pediatric use. The compatibility between drug and possible excipients were evaluated by differential scanning calorimetry (DSC) and the captopril thermic behavior, through thermogravimetric analysis (TG) and differential thermal analysis (DTA). Then, particle size and indirect flow measures of captopril and excipients were analyzed. For solution studies, different formulations were obtained through factorial design, varying the EDTA concentration (0.005 and 0.1%) and pH (2.5, 4.0 e 5.5) in distilled and mineral water, which were stored at 60°C and analyzed over twelve days by HPLC to evaluate the stability of captopril. In the DSC curves of captopril mixtures with preservatives, sucralose and citric acid, the isolated thermal events were not maintained. In the other binary mixtures, the events corresponding to each component were preserved in the curves, indicating compatibility between substances. There was a major difference in the distribution and average particles diameters and density of buffering agents in comparison to other substances, which can cause segregation of the powder mixture. From the study of the solutions stability it was found that the variables interfere significantly (p = 0.05) in the captopril content, the pH being the most important factor. The interactions between variables were significant, with greater stability around pH 4.0, higher EDTA concentrations and use of mineral water. Based on the results, it can be concluded that development of a stable captopril formulation is viable if strategic measures are adopted in order to avoid segregation of the powders constituents of the formulation.Master Thesis Desenvolvimento e caracterização de nanoemulsões cosméticas contendo extrato de Opuntia ficus-indica L. Mill e avaliação in vivo da eficácia hidratante(Universidade Federal do Rio Grande do Norte, 2015-03-12) Ribeiro, Renato César de Azevedo; Ferrari, Márcio; Veríssimo, Lourena Mafra; ; http://lattes.cnpq.br/9173400981540256; ; http://lattes.cnpq.br/5782539548696465; ; http://lattes.cnpq.br/7437846439578166; Egito, Eryvaldo Sócrates Tabosa do; ; http://lattes.cnpq.br/6907806915889763; Campos, Patrícia Maria Berardo Gonçalves Maia; ; http://lattes.cnpq.br/1103573381816124Nanoemulsions are emulsified systems, characterized for reduced droplet size (50- 500nm), which the main characteristic are kinect stability and thermodynamic instability. These are promising systems on cosmetic area due to their droplet size that provide different advantages when compared to conventional systems, among others, larger surface area and better permeability. The Opuntia ficus-indica (L.) Mill is a plant cultivated on Caatinga Brazilian biome, which has great socioeconomic importance to region. This plant shows carbohydrates utilized for cosmetic industry as moisturizing active in their chemical composition. The aim of study was to develop, characterize, evaluate stability and moisturizing efficacy of cosmetic nanoemulsions added to Opuntia ficus-indica (L.) Mill extract. Nanoemulsions preparation was made using a low energy method. Different nanoemulsions were formulated varying the ratio of oil, water and surfactant phases beyond xanthan gum (0.5% e 1%) and Opuntia ficus-indica (L.) Mill hydroglycolic extract addition on 1% and 3%. Obtained nanoemulsions were submitted to preliminary and accelerated stability tests. The evaluated parameters monitored were: macroscopic aspect, pH value, droplet size, zeta potential and polydispersion index, during 60 days on different temperatures. Stable formulations were submitted to moisturizing efficacy assessment by capacitance and transepidermal water loss methodologies during 5 hours. Stable samples were white and showed homogeneous and fluid aspect, pH value was inside ideal range (4,5-6,0) to topical application and droplet size under 200nm characterizing these system as nanoemulsions. Developed nanoemulsions did not decrease transepidermal water loss, however increased the water content on stratum corneum, highlighting the nanoemulsions containing 0.5% of xanthan gum and 1% of hydroglycolic extract. This work presents cosmetic moisturizing nanoemulsions composed to vegetal raw material from Brazilian Caatinga with potential to be used on cosmetic area.Master Thesis Desenvolvimento de emulsões contendo óleos vegetais para uso cosmético(Universidade Federal do Rio Grande do Norte, 2009-09-28) Farias, Iury Einstein Gomes de; ; http://lattes.cnpq.br/6907806915889763; ; http://lattes.cnpq.br/1755174854925410; Nagashima Junior, Toshiyuki; ; Lima, Eliana Martins; ; http://lattes.cnpq.br/7248774319455970The fat acid esters and tocopherolic derivatives are of great economic interest in many industries. The sunflower oil, which had its rich constitution in these composites, is a very interesting raw material source for the job in some sectors as bio-carburants, bio-lubrificants, bio-surfactants, dispersing agents, food industries, medicines and cosmetics. A system emulsified steady from this oil can wide be used in the therapeutical one, therefore it is of easy acceptance for the patient, for being pharmaceutical forms that allow a better medicine administration. The chemical composition characteristics, rich in unsaturad fat acid and tocopherolic derivatives, the sunflower oil, make of the emulsified systems contend this oil a proposal promising for formularizations of pharmaceutical and cosmetic use with antirust and photoprotection. The general objective of this work was to apply the HLB beddings to determine the sunflower oil critical HLB and, from this, to be able to evaluate the ideal mixture of the constituent of this system through the study of the ternary diagrams for the determination of the ratio of constituent that will generate the emulsion most steady