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https://repositorio.ufrn.br/handle/123456789/21750| Title: | Comparative analysis of Enterococcus faecalis biofilm formation on different substrates |
| Authors: | Guerreiro-Tanomaru, Juliane Maria Faria Júnior, Norberto Batista de Duarte, Marco Antonio Hungaro Ordinola-Zapata, Ronald Tanomaru-Filho, Mário |
| Keywords: | Biofilm;Confocal microscopy;Enterococcus faecalis |
| Issue Date: | 2013 |
| Citation: | GUERREIRO-TANOMARU, Juliane Maria et al. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. Journal of Endodontics , v. 39, n. 3, p. 346-350, 2013. |
| Portuguese Abstract: | INTRODUCTION: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. METHODS: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37°C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (μm(3)), live bacteria biovolume (μm(3)), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05). RESULTS: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05). CONCLUSIONS: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated. |
| URI: | https://repositorio.ufrn.br/jspui/handle/123456789/21750 |
| Appears in Collections: | CCS - DOD - Artigos publicados em periódicos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ComparativeAnalysisEnterococcus_GuerreiroTanomaru_2013.pdf | https://www.ncbi.nlm.nih.gov/pubmed/23402505 | 883,89 kB | Adobe PDF | View/Open |
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