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Title: Comparative analysis of Enterococcus faecalis biofilm formation on different substrates
Authors: Guerreiro-Tanomaru, Juliane Maria
Faria Júnior, Norberto Batista de
Duarte, Marco Antonio Hungaro
Ordinola-Zapata, Ronald
Tanomaru-Filho, Mário
Keywords: Biofilm;Confocal microscopy;Enterococcus faecalis
Issue Date: 2013
Citation: GUERREIRO-TANOMARU, Juliane Maria et al. Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. Journal of Endodontics , v. 39, n. 3, p. 346-350, 2013.
Portuguese Abstract: INTRODUCTION: The aim of this study was to compare Enterococcus faecalis biofilm formation on different substrates. METHODS: Cell culture plates containing growth medium and E. faecalis (ATCC 29212) were used to grow biofilm on bovine dentin, gutta-percha, hydroxyapatite, or bovine bone. Substrates were incubated at 37°C for 14 or 21 days, and the medium was changed every 48 hours. After the growth induction periods, specimens (n = 5 per group and per induction period) were stained by using Live/Dead, and the images were analyzed under a confocal microscope. The total biovolume (μm(3)), live bacteria biovolume (μm(3)), and substrate coverage (%) were quantified by using the BioImage_L software. Results obtained were analyzed by nonparametric tests (P = .05). RESULTS: Biofilm formation was observed in all groups. Gutta-percha had the lowest total biovolume at 14 days (P < .05) and hydroxyapatite the highest at 21 days (P < .05). No significant difference was observed in green biovolume at 14 days. At 21 days, however, hydroxyapatite had the highest volume (P < .05). The percentages of coverage were similar among all substrates at 21 days (P > .05), but at 14 days, bovine bone presented the highest coverage (P < .05). CONCLUSIONS: E. faecalis was capable of forming biofilm on all substrates during both growth periods; hydroxyapatite presented the highest rates of biofilm formation. The type of substrate influenced the biofilm characteristics, according to the parameters evaluated.
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