Please use this identifier to cite or link to this item:
Title: Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes
Authors: Chouchane, Malek
Faria, Ana Raquel Melo de
Moura, Daniela Maria de Souza
Hilscher, Markus Michael
Schroder, Timm
Leão, Richardson Naves
Costa, Marcos Romualdo
Keywords: Lineage reprogramming;Induced neurons;Proneural genes;Astroglial cells;Cerebral cortex;Cerebellum;Cell transplantation
Issue Date: Jul-2017
Portuguese Abstract: Astroglial cells isolated from the rodent postnatal cerebral cortex are particularly susceptible to lineage reprogramming into neurons. However, it remains unknown whether other astroglial populations retain the same potential. Likewise, little is known about the fate of induced neurons (iNs) in vivo. In this study we addressed these questions using two different astroglial populations isolated from the postnatal brain reprogrammed either with Neurogenin-2 (Neurog2) or Achaete scute homolog-1 (Ascl1). We show that cerebellum (CerebAstro) and cerebral cortex astroglia (CtxAstro) generates iNs with distinctive neurochemical and morphological properties. Both astroglial populations contribute iNs to the olfactory bulb following transplantation in the postnatal and adult mouse subventricular zone. However, only CtxAstro transfected with Neurog2 differentiate into pyramidal-like iNs after transplantation in the postnatal cerebral cortex. Altogether, our data indicate that the origin of the astroglial population and transcription factors used for reprogramming, as well as the region of integration, affect the fate of iNs.
ISSN: 2213-6711
Appears in Collections:ICe - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
lineage reprogramming.pdfArtigo completo5,11 MBAdobe PDFThumbnail

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.