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Title: Protective effect of dexamethasone on 5-FU-induced oral mucositis in hamsters
Authors: Araújo, Aurigena Antunes de
Ribeiro, Susana Barbosa
Araújo Júnior, Raimundo Fernandes de
Brito, Gerly Anne de Castro
Leitão, Renata Carvalho
Barbosa, Maisie Mitchele
Garcia, Vinicius Barreto
Medeiros, Aldo Cunha
Medeiros, Caroline Addison Carvalho Xavier de
Keywords: dexamethasone
Issue Date: 23-Oct-2017
Publisher: Augusta University, UNITED STATES
Citation: ARAÚJO, Aurigena Antunes de et al.Protective effect of dexamethasone on 5-FU-induced oral mucositis in hamsters. PLoS One, v. 12, p. e0186511-xx, 2017. Disponível em: <>. Acesso em: 20 mar. 2018.
Portuguese Abstract: Oral mucositis (OM) is an important side effect of cancer treatment, characterized by ulcerative lesions in the mucosa of patients undergoing radiotherapy or chemotherapy, which has marked effects on patient quality of life and cancer therapy continuity. Considering that few protocols have demonstrated efficacy in preventing this side effect, the aim of this study was to examine the effect of dexamethasone (DEX) on OM induced by 5-fluorouracil (5-FU) in hamsters by studying signaling pathways. OM was induced in hamsters by 5-FU followed by mechanical trauma (MT) on day 4. On day 10, the animals were euthanized. The experimental groups included saline, MT, 5-FU, and DEX (0.25, 0.5, or 1 mg/kg). Macroscopic, histopathological, and immunohistochemical analyses as well as immunofluorescence experiments were performed on the oral mucosa of the animals. The oral mucosal samples were analyzed by enzyme-linked immunosorbent assays, and quantitative real-time polymerase chain reaction (qPCR). DEX (0.5 or 1 mg/kg) reduced inflammation and ulceration of the oral mucosa of hamsters. In addition, DEX (1 mg/kg) reduced the cytokine levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and macrophage migration inhibitory factor (MIF). DEX (1 mg/kg) also reduced the immunoexpression of cyclooxygenase (COX)-2, matrix metalloproteinase (MMP)-2, transforming growth factor (TGF)-β, MIF, Smad 2/3, Smad 2/3 phosphorylated and NFκB p65 in the jugal mucosa. Finally, DEX (1 mg/kg) increased interleukin-1 receptor-associated kinase 3 (IRAK-M), glucocorticoid-induced leucine zipper (GILZ), and mitogen-activated protein kinase (MKP1) gene expression and reduced NFκB p65 and serine threonine kinase (AKt) gene expression, relative to the 5-FU group. Thus, DEX improved OM induced by 5-FU in hamsters.
ISSN: 1932-6203
Appears in Collections:CB - DBF - Artigos publicados em periódicos

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