Please use this identifier to cite or link to this item:
Title: Pyk2 overexpression in postsynaptic neurons blocks amyloid β1-42-induced synaptotoxicity in microfluidic co-cultures
Authors: Kilinc, Devrim
Vreulx, Anaїs-Camille
Mendes, Tiago
Flaig, Amandine
Coelho, Diego Marques
Verschoore, Maxime
Demiautte, Florie
Amouyel, Philippe
Eysert, Fanny
Dourlen, Pierre
Chapuis, Julien
Costa, Marcos Romualdo
Malmanche, Nicolas
Checler, Frédéric
Lambert, Jean-Charles
Keywords: Alzheimer disease;Synapses;Microfluidics;Coculture techniques;Amyloid beta-peptides;Amyloid beta-protein precursor
Issue Date: 28-Aug-2020
Publisher: Oxford University Press
Citation: KILINC, Devrim et al. Pyk2 overexpression in postsynaptic neurons blocks amyloid β1-42-induced synaptotoxicity in microfluidic co-cultures. Brain Communications, [S.l.], fcaa139, ago. 2020. Disponível em: Acesso em: 1 set. 2020.
Portuguese Abstract: Recent meta-analyses of genome-wide association studies identified a number of genetic risk factors of Alzheimer’s disease; however, little is known about the mechanisms by which they contribute to the pathological process. As synapse loss is observed at the earliest stage of Alzheimer’s disease, deciphering the impact of Alzheimer’s risk genes on synapse formation and maintenance is of great interest. In this paper, we report a microfluidic co-culture device that physically isolates synapses from pre- and postsynaptic neurons and chronically exposes them to toxic amyloid β peptides secreted by model cell lines overexpressing wild-type or mutated (V717I) amyloid precursor protein. Co-culture with cells overexpressing mutated amyloid precursor protein exposed the synapses of primary hippocampal neurons to amyloid β1-42 molecules at nanomolar concentrations and induced a significant decrease in synaptic connectivity, as evidenced by distance-based assignment of postsynaptic puncta to presynaptic puncta. Treating the cells with antibodies that target different forms of amyloid β suggested that low molecular weight oligomers are the likely culprit. As proof of concept, we demonstrate that overexpression of protein tyrosine kinase 2 beta (Pyk2) –an Alzheimer’s disease genetic risk factor involved in synaptic plasticity and shown to decrease in Alzheimer’s disease brains at gene expression and protein levels– selectively in postsynaptic neurons is protective against amyloid β1-42-induced synaptotoxicity. In summary, our lab-on-a-chip device provides a physiologically-relevant model of Alzheimer’s disease-related synaptotoxicity, optimal for assessing the impact of risk genes in pre- and postsynaptic compartments
Appears in Collections:ICe - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
Pyk2OverexpressionPostsynaptic_Costa_2020.pdfPyk2OverexpressionPostsynaptic_Costa_20203.26 MBAdobe PDFThumbnail

This item is licensed under a Creative Commons License Creative Commons