Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/handle/123456789/31159
Title: A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro
Authors: Menezes, Maira Maria
Nobre, Leonardo Thiago Duarte Barreto
Rossi, Gustavo Rodrigues
Almeida-Lima, Jailma
Melo-Silveira, Raniere Fagundes
Franco, Celia Regina Cavichiolo
Trindade, Edvaldo Silva
Nader, Helena Bonciani
Rocha, Hugo Alexandre de Oliveira
Keywords: Fucoidan;Brown seaweed, endothelial cells;Anti-angiogenic
Issue Date: May-2018
Publisher: Elsevier
Citation: MENEZES, Maira Maria; NOBRE, Leonardo Thiago Duarte Barreto; ROSSI, Gustavo Rodrigues; ALMEIDA-LIMA, Jailma; MELO-SILVEIRA, Raniere Fagundes; FRANCO, Celia Regina Cavichiolo; TRINDADE, Edvaldo Silva; NADER, Helena Bonciani; ROCHA, Hugo Alexandre de Oliveira. A low-molecular-weight galactofucan from the seaweed, Spatoglossum schröederi, binds fibronectin and inhibits capillary-like tube formation in vitro. International Journal Of Biological Macromolecules, [s. l.], v. 111, p. 1067-1075, maio 2018. Elsevier BV. Disponível em: https://www.sciencedirect.com/science/article/pii/S0141813017333792?via%3Dihub. Acesso em: 18 set. 2020.http://dx.doi.org/10.1016/j.ijbiomac.2018.01.119.
Portuguese Abstract: A low-molecular-weight (LMW) heterofucan (designated fucan B) was obtained from the brown seaweed, Spatoglossum schröederi, and its activity as an inhibitor of capillary-like tube formation by endothelial cells (ECs) was analyzed. Chemical, infrared and electrophoretic analyses confirmed the identity of fucan B. In contrast to other LMW fucans, fucan B (0.012–0.1 mg/mL) inhibited ECs capillary-like tube formation in a concentrationdependent manner. In addition, fucan B (0.01–0.05 mg/mL) did not affect ECs proliferation. Fucan B also inhibited ECs migration on a fibronectin-coated surface, but not on laminin- or collagen-coated surfaces. Biotinylated fucan B was used as a probe to identify its localization. Confocal microscopy experiments revealed that biotinylated fucan did not bind to the cell surface, but rather only to fibronectin. Our findings suggest that fucan B inhibits ECs capillary-like tube formation and migration by binding directly to fibronectin and blocking fibronectin sites recognized by cell surface ligands. However, further studies are needed to evaluate the in vivo effects of fucan B
URI: https://repositorio.ufrn.br/handle/123456789/31159
ISSN: 0141-8130 (print)
Appears in Collections:CB - DBQ - Artigos publicados em periódicos
EMCM - Artigos publicados em periódicos

Files in This Item:
File Description SizeFormat 
LowMolecularWeight_Nobre_2018.pdf2,9 MBAdobe PDFThumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons