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Title: Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae
Authors: Fidelis, Gabriel Pereira
Câmara, Rafael Barros Gomes da
Queiroz, Moacir Fernandes
Costa, Mariana Santana Santos Pereira
Santos, Pablo Castro
Rocha, Hugo Alexandre de Oliveira
Costa, Leandro Silva
Keywords: APTT assay;Total capacity antioxidant;Sonication;Proteolysis;Red seaweed
Issue Date: 13-Nov-2014
Publisher: MDPI
Citation: FIDELIS, Gabriel Pereira; CAMARA, Rafael Barros Gomes da; QUEIROZ, Moacir Fernandes; COSTA, Mariana Santana Santos Pereira; SANTOS, Pablo Castro; ROCHA, Hugo Alexandre de Oliveira; COSTA, Leandro Silva. Proteolysis, NaOH and ultrasound-enhanced extraction of anticoagulant and antioxidant sulfated polysaccharides from the edible seaweed, Gracilaria birdiae. Molecules, [s. l.], v. 19, n. 11, p. 18511-18526, 13 nov. 2014. MDPI AG. Disponível em: Acesso em: 05 ago. 2020. Http://
Portuguese Abstract: The sulfated polysaccharides (SP) from the edible red seaweed, Gracilaria birdiae, were obtained using five different extraction conditions: Gracilaria birdiae 1 (GB1)-water; GB1s-water/sonication; GB1sp-water/sonication/proteolysis; GB2s-NaOH/sonication; and GB2sp-NaOH/sonication/proteolysis. The yield (g) increased in the following order: GB2sp > GB1sp > GB2s > GB1s > GB1. However, the amount of SP extracted increased in a different way: GB2sp > GB1 > GB1sp > GB1s > GB2s. Infrared and electrophoresis analysis showed that all conditions extracted the same SP. In addition, monosaccharide composition showed that ultrasound promotes the extraction of polysaccharides other than SP. In the prothrombin time (PT) test, which evaluates the extrinsic coagulation pathway, none of the samples showed anticoagulant activity. While in the activated partial thromboplastin time (aPTT) test, which evaluates the intrinsic coagulation pathway, all samples showed anticoagulant activity, except GB2s. The aPTT activity decreased in the order of GB1sp > GB2sp > GB1 > GB1s > GB2s. The total capacity antioxidant (TCA) of the SP was also affected by extraction condition, since GB2s and GB1 showed lower activity in comparison to the other conditions. In conclusion, the conditions of SP extraction influence their biological activities and chemical composition. The data revealed that NaOH/sonication/proteolysis was the best condition to extract anticoagulant and antioxidant SPs from Gracilaria birdiae
ISSN: 1420-3049 (online)
Appears in Collections:CB - DBQ - Artigos publicados em periódicos
EMCM - Artigos publicados em periódicos

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