Please use this identifier to cite or link to this item: https://repositorio.ufrn.br/handle/123456789/31829
Title: Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium
Other Titles: Expansión y cambios fenotípicos de células mesenquimales de médula ósea de ratón cultivadas con FGF-2 y medio condicionado por el nervio facial
Authors: Lucena, Eudes Euler de Souza
Morais, Hécio Henrique Araújo de
Araújo, Dayane Pessoa de
Cavalcanti, José Rodolfo Lopes de Paiva
Azevedo, Eduardo Pereira de
Queiroz, Dinalva Brito de
Botelho, Marco Antônio
Rêgo, Amália Cinthia Meneses do
Araújo Filho, Irami
Barboza, Carlos Augusto Galvão
Nascimento Júnior, Expedito Silva do
Costa, Miriam Stela Maris de Oliveira
Cavalcante, Jeferson de Sousa
Guzen, Fausto Pierdoná
Keywords: Mesenchymal stem cells;Facial nerve injury;Fibroblast growth factor 2;Conditioned medium;Plasticity;Trans-differentiation
Issue Date: Sep-2018
Publisher: Sociedade Chilena de Anatomía
Citation: LUCENA, Eudes Euler de Souza; MORAIS, Hécio Henrique Araújo de; ARAÚJO, Dayane Pessoa de; CAVALCANTI, José Rodolfo Lopes de Paiva; AZEVEDO, Eduardo Pereira de; QUEIROZ, Dinalva Brito de; BOTELHO, Marco Antônio; REGO, Amália Cinthia Meneses do; ARAÚJO FILHO, Irami; BARBOZA, Carlos Augusto Galvão; NASCIMENTO JUNIOR, Expedito Silva do; COSTA, Miriam Stela Maris de Oliveira; CAVALCANTE, Jeferson de Souza. Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium. International Journal Of Morphology, [s. l.], v. 36, n. 3, p. 1049-1056, set. 2018. SciELO Agencia Nacional de Investigacion y Desarrollo (ANID). Disponível em: https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-95022018000301049&lng=pt&nrm=iso&tlng=en. Acesso em: 20 jul. 2020. http://dx.doi.org/10.4067/s0717-95022018000301049
Portuguese Abstract: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation
URI: https://repositorio.ufrn.br/handle/123456789/31829
ISSN: 0717-9367 (print)
0717-9502 (0nline)
Appears in Collections:CB - DFIS - Artigos publicados em periódicos
CB - DMOR - Artigos publicados em periódicos
CCS - DCIRUR - Artigos publicados em periódicos
EMCM - Artigos publicados em periódicos

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