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https://repositorio.ufrn.br/handle/123456789/31829| Title: | Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium |
| Other Titles: | Expansión y cambios fenotípicos de células mesenquimales de médula ósea de ratón cultivadas con FGF-2 y medio condicionado por el nervio facial |
| Authors: | Lucena, Eudes Euler de Souza Morais, Hécio Henrique Araújo de Araújo, Dayane Pessoa de Cavalcanti, José Rodolfo Lopes de Paiva Azevedo, Eduardo Pereira de Queiroz, Dinalva Brito de Botelho, Marco Antônio Rêgo, Amália Cinthia Meneses do Araújo Filho, Irami Barboza, Carlos Augusto Galvão Nascimento Júnior, Expedito Silva do Costa, Miriam Stela Maris de Oliveira Cavalcante, Jeferson de Sousa Guzen, Fausto Pierdoná |
| Keywords: | Mesenchymal stem cells;Facial nerve injury;Fibroblast growth factor 2;Conditioned medium;Plasticity;Trans-differentiation |
| Issue Date: | Sep-2018 |
| Publisher: | Sociedade Chilena de Anatomía |
| Citation: | LUCENA, Eudes Euler de Souza et al. Expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with FGF-2 and facial nerve-conditioned medium. International Journal Of Morphology, [s. l.], v. 36, n. 3, p. 1049-1056, set. 2018. SciELO Agencia Nacional de Investigacion y Desarrollo (ANID). Disponível em: https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-95022018000301049&lng=pt&nrm=iso&tlng=en. Acesso em: 20 jul. 2020. http://dx.doi.org/10.4067/s0717-95022018000301049 |
| Portuguese Abstract: | Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation |
| URI: | https://repositorio.ufrn.br/handle/123456789/31829 |
| ISSN: | 0717-9367 (print) 0717-9502 (0nline) |
| Appears in Collections: | CB - DFIS - Artigos publicados em periódicos CB - DMOR - Artigos publicados em periódicos CCS - DCIRUR - Artigos publicados em periódicos EMCM - Artigos publicados em periódicos |
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| File | Description | Size | Format | |
|---|---|---|---|---|
| PhenotypicMesenchymalFacial_Lucena_2018.pdf | 334,88 kB | Adobe PDF |  View/Open |
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