Uso de corante natural extraído do pau-brasil (Paubrasilia echinata) para o processo de plastinação

Abstract

Anatomy, known for several centuries, is an extremely important discipline in the training of health professionals. The use of cadavers, which is essential for teaching, leads to broad discussions about the most efficient way to use these pieces. Currently, the most common way to preserve cadaveric pieces for teaching anatomy is to use formaldehyde, “a volatile chemical substance with an unpleasant odor, which causes irritation in the eyes and respiratory tract”. Seeking to further popularize and expand the teaching of anatomy, the German doctor Dr. Von Hagens developed a technique in 1977 called Plastination, which favors the minimization of unwanted effects caused by formaldehyde The technique consists of a dehydration process, seeking to remove fatty tissue and water present in the piece, followed by the impregnation of polymers: silicone, epoxy and/or polyester in biological tissues, which allows for greater durability of cadaveric pieces. Even though it is an innovative technique and facilitates the study of anatomy, even though it is an innovative technique, there are possible improvements to be made with regard to color that suffers natural loss. This technique allows for greater durability of cadaveric pieces and makes them appear closer to natural. As it does not contain preservative substances that cause harm to health, plastination facilitates the handling and use of cadaveric parts for teaching and scientific purposes. To make the visualization of the structures even more evident, specific dyes can be used, in an attempt to bring the plastinated pieces even closer to the real thing, such as painting the pieces. As a result, it was necessary to develop a protocol for staining muscle tissues, associated with the plastination technique, making the technique even more efficient. According to what has been explained, the objective of the present work is to analyze the quality of coloring in pieces plastinated with dye extracted from the bark of Brazilwood (Paubrasilia echinata) in the plastination technique. For this, dye allocation methods were used in different stages of the plastination process, as well as its association with different dehydrating substances, in which acetone or ethyl alcohol will be used, in order to evaluate the best efficiency of the dye for producing the protocol, adapting to the plastination process. Which will make teaching human anatomy more efficient.