A serine proteinase inhibitor isolated from Tamarindus indica seeds and its effects on the release of human neutrophil elastase

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dc.contributor.authorQueiroz, Alexandre Flavio Silva de
dc.contributor.authorFook, J.M.S.L.L.
dc.contributor.authorMacedo, L.L.P.
dc.contributor.authorMoura, G.E.D.D.
dc.contributor.authorTeixeira, F.M.
dc.contributor.authorOliveira, A.S.
dc.contributor.authorSales, M.P.
dc.date.accessioned2018-05-28T18:11:52Z
dc.date.available2018-05-28T18:11:52Z
dc.date.issued2005
dc.description.resumoProteinaceous inhibitors with high inhibitory activities against human neutrophil elastase (HNE) were found in seeds of the Tamarind tree (Tamarindus indica). A serine proteinase inhibitor denoted PG50 was purified using ammonium sulphate and acetone precipitation followed by Sephacryl S-300 and Sephadex G-50 gel filtration chromatographies. Inhibitor PG50 showed a Mr of 14.9 K on Sephadex G-50 calibrated column and a Mr of 11.6 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. PG50 had selective activity while cysteine proteinases (papain and bromelain) and serine proteinases (porcine pancreatic elastase and bovine chymotrypsin) were not inhibited, it was strongly effective against serine proteinases such as bovine trypsin and isolated human neutrophil elastase. The IC50 value was determined to be 55.96 Ag.mL_1. PG50 showed neither cytotoxic nor haemolytic activity on human blood cells. After pre-incubation of PG50 with cytochalasin B, the exocytosis of elastase was initiated using PAF and fMLP. PG50 exhibited different inhibition on elastase release by PAF, at 44.6% and on release by fMLP, at 28.4%. These results showed that PG50 preferentially affected elastase release by PAF stimuli and this may indicate selective inhibition on PAF receptors.pt_BR
dc.identifier.citationQueiroz,A.F.S.; Fook,J.M.S.L.L.; Macedo,L.L.P.; Moura,G.E.D.D.; Teixeira,F.M.; Oliveira,A.S.; Sales,M.P. A serine proteinase inhibitor isolated from tamarindus indica seeds and its effects on the realese of human neutrophil elastase. Life Sciences, v. 76, n.1, p. 2881-2891, 2005. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0024320505000792> Acesso em: 2 mar. 2018.pt_BR
dc.identifier.doi10.1016/j.lfs.2004.10.053
dc.identifier.issn1879-0631
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/25252
dc.languageengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectTamarindus indicapt_BR
dc.subjectHuman neutrophil elastasept_BR
dc.subjectPAFpt_BR
dc.subjectfMLPpt_BR
dc.titleA serine proteinase inhibitor isolated from Tamarindus indica seeds and its effects on the release of human neutrophil elastasept_BR
dc.typearticlept_BR

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