Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography

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dc.contributor.authorSantos, Everaldo Silvino dos
dc.contributor.authorSousa Junior, Francisco Canindé de
dc.contributor.authorVaz, Michelle Rossana Ferreira
dc.contributor.authorPadilha, Carlos Eduardo de Araújo
dc.contributor.authorMartins, Daniella Regina Arantes
dc.contributor.authorMacedo, Gorete Ribeiro de
dc.date.accessioned2021-05-21T01:52:31Z
dc.date.available2021-05-21T01:52:31Z
dc.date.issued2014-10-01
dc.description.resumoVisceral leishmaniasis, a disease caused by Leishmania infantum chagasi, represents a major public health problem in many areas of the world. Despite the considerable effort, there is no effective and safe vaccine for human use [1]. Some authors have reported that as much as 50% of overall costs in the biotechnology industries are related to downstream processing. Thus, the development of new and economically advantageous purification methods is a challenge [2]. Expanded bed adsorption (EBA) is an innovative chromatography technology that allows the adsorption of target proteins directly from unclarified feedstock. EBA technology combines solid-liquid separation with an adsorption step in a single-unit operation, aiming at increased overall yield, reduced operational time, and less capital investment and consumables [3,4]. Thus, the aim of this work was to purify the 503 antigen of Leishmania i. chagasi directly from crude feedstock using EBA chromatographypt_BR
dc.identifier.citationSOUSA JUNIOR, Francisco Canindé de; VAZ, Michelle Rossana Ferreira; PADILHA, Carlos Eduardo de Araújo; MARTINS, Daniella Regina Arantes; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Production, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatography. Bmc Proceedings, [S.L.], v. 8, n. 4, out. 2014. Disponível em: https://bmcproc.biomedcentral.com/articles/10.1186/1753-6561-8-S4-P194. Acesso em: 08 abr. 2021. https://doi.org/10.1186/1753-6561-8-S4-P194pt_BR
dc.identifier.doi10.1186/1753-6561-8-S4-P194
dc.identifier.issn1753-6561
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/32543
dc.languageenpt_BR
dc.publisherBMCpt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectVisceral Leishmaniasispt_BR
dc.subjectLeishmaniasispt_BR
dc.subjectElution Conditionpt_BR
dc.subjectBatch Adsorption Experimentpt_BR
dc.subjectLeishmania Infantumpt_BR
dc.titleProduction, recovery, and purification of recombinant 503 antigen of Leishmania infantum chagasi using expanded bed adsorption chromatographypt_BR
dc.typearticlept_BR

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