Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing

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dc.contributor.authorSantos, Yago Queiroz dos
dc.contributor.authorFrança, Anderson Felipe Jácome de
dc.contributor.authorVeras, Bruno Oliveira de
dc.contributor.authorCarelli, Gabriella Silva Campos
dc.contributor.authorMoura, Geovanna Maria Medeiros
dc.contributor.authorCruz, Joelton Igor Oliveira da
dc.contributor.authorOliveira, Fernanda Granja da Silva
dc.contributor.authorOliveira, João Ricardhis Saturnino de
dc.contributor.authorAmorim, Luciclaudio Cassimiro de
dc.contributor.authorSantos, Elizeu Antunes dos
dc.date.accessioned2021-07-05T13:33:48Z
dc.date.available2021-07-05T13:33:48Z
dc.date.issued2019
dc.description.resumoThe microorganisms belonging to genus Bacillus include a wide spectra and ubiquitous group of bacteria that can be found from the forest soil, to marine ecosystems occurring in association with a variety of aquatic organisms such as scleractinian corals present in the intertidal boulders. Therefore, these microorganisms are exposed to various abiotic stresses that cause ecological selection for a physiologically adapted microbiota to such extremes of temperature and salinity. In this work, we isolated and characterized a cellulase from a marine bacterial strain and indentified its specie by 16S rRNA sequencing followed by a BLAST analysis. The cellulolytic strain called SR22 showed to be a gram-positive spore-forming bacilli, facultative anaerobe, and catalase positive, as well as negative for indole, H2S production, and citrate utilization; those findings led us to consider the isolate belonging to the genus Bacillus, which was confirmed by the phylogenetic analysis, which revealed that the SR22 strain formed a clade with Bacillus subtilis. Its nucleotide sequence was deposited in GenBank as Accession No. MH119099 and the degree of sequence similarity of strain SR22 to Bacillus sp. was 99%. Taken together, the present data indicate the present celulase-positive indentified strain as a potential and useful candidate for industrial applications that employs celulase degrading processes like second-generation bioethanol and paper industries being still necessary further studies to complete characterize this microorganism secretomept_BR
dc.identifier.citationSANTOS, Yago Queiroz dos et al. Phylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencing. In: SILVA NETO, Benedito Rodrigues da (Org.). Conceitos Básicos da Genética. 1. ed. Ponta Grossa, PR: Atena Editora, 2019. p. 202-209pt_BR
dc.identifier.doi10.22533/at.ed.214192106
dc.identifier.isbn978-85-7247-421-4
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/32805
dc.languageenpt_BR
dc.publisherAtena Editorapt_BR
dc.subjectEndoglucanasept_BR
dc.subjectMarine bacteriapt_BR
dc.subjectrRNApt_BR
dc.titlePhylogenetic analysis and identification of a cellulase producing Bacillus Sp. Strain by 16s RRNA sequencingpt_BR
dc.typearticlept_BR

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