Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis

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dc.contributor.authorAraújo, Aurigena Antunes de
dc.contributor.authorPereira, Aline de Sousa Barbosa Freitas
dc.contributor.authorMedeiros, Caroline Addison Carvalho Xavier de
dc.contributor.authorBrito, Gerly Anne de Castro
dc.contributor.authorLeitão, Renata Ferreira de Carvalho
dc.contributor.authorAraújo, Lorena de Souza
dc.contributor.authorGuedes, Paulo Marcos Matta
dc.contributor.authorHiyari, Sarah
dc.contributor.authorPirih, Flávia Q.
dc.contributor.authorAraújo Júnior, Raimundo Fernandes de
dc.date.accessioned2020-09-28T17:13:18Z
dc.date.available2020-09-28T17:13:18Z
dc.date.issued2017-08-28
dc.description.resumoAim To evaluate the effects of metformin (Met) on inflammation, oxidative stress, and bone loss in a rat model of ligature-induced periodontitis. Materials & methods Male albino Wistar rats were divided randomly into five groups of twenty-one rats each, and given the following treatments for 10 days: (1) no ligature + water, (2) ligature + water, (3) ligature + 50 mg/kg Met, (4) ligature + 100 mg/kg Met, and (5) ligature + 200 mg/kg Met. Water or Met was administered orally. Maxillae were fixed and scanned using Micro-computed Tomography (μCT) to quantitate linear and bone volume/tissue volume (BV/TV) volumetric bone loss. Histopathological characteristics were assessed through immunohistochemical staining for MMP-9, COX-2, the RANKL/RANK/OPG pathway, SOD-1, and GPx-1. Additionally, confocal microscopy was used to analyze osteocalcin fluorescence. UV-VIS analysis was used to examine the levels of malondialdehyde, glutathione, IL-1β and TNF-α from gingival tissues. Quantitative RT-PCR reaction was used to gene expression of AMPK, NF-κB (p65), and Hmgb1 from gingival tissues. Significance among groups were analysed using a one-way ANOVA. A p-value of p<0.05 indicated a significant difference. Results Treatment with 50 mg/kg Met significantly reduced concentrations of malondialdehyde, IL-1β, and TNF-α (p < 0.05). Additionally, weak staining was observed for COX-2, MMP-9, RANK, RANKL, SOD-1, and GPx-1 after 50 mg/kg Met. OPG and Osteocalcin showed strong staining in the same group. Radiographically, linear measurements showed a statistically significant reduction in bone loss after 50 mg/kg Met compared to the ligature and Met 200 mg/kg groups. The same pattern was observed volumetrically in BV/TV and decreased osteoclast number (p<0.05). RT-PCR showed increased AMPK expression and decreased expression of NF-κB (p65) and HMGB1 after 50 mg/kg Met. Conclusions Metformin, at a concentration of 50 mg/kg, decreases the inflammatory response, oxidative stress and bone loss in ligature-induced periodontitis in rats.pt_BR
dc.identifier.citationARAÚJO, Aurigena Antunes de et al. Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One, v. 12, p. 1-21, 2017. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0183506>. Acesso em: 21 mar. 2018. https://doi.org/10.1371/journal.pone.0183506pt_BR
dc.identifier.doi10.1371/journal.pone.0183506
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/30219
dc.languagept_BRpt_BR
dc.publisherCharles P. Darby Children's Research Institute, UNITED STATESpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectOxidative stresspt_BR
dc.subjectMetformin on inflammationpt_BR
dc.titleEffects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitispt_BR
dc.typearticlept_BR

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