Near infrared spectroscopy and multivariate calibration for simultaneous determination of glucose, triglycerides and high-density lipoprotein in animal plasma

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dc.contributor.authorAraújo, Aurigena Antunes de
dc.contributor.authorNeves, Ana Carolina de Oliveira
dc.contributor.authorSilva, Bruna Laís
dc.contributor.authorValderrama, Patrícia
dc.contributor.authorMarc, Paulo Henrique
dc.contributor.authorLima, Kássio Michell Gomes de
dc.date.accessioned2018-06-16T11:28:15Z
dc.date.available2018-06-16T11:28:15Z
dc.date.issued2012
dc.description.resumoThe quantitative analysis of glucose, triglycerides and high-density lipoprotein (HDL) in rat plasma without sample pre-treatment using direct near-infrared spectroscopy was studied. Comparison was made of several multivariate calibration techniques and algorithms for data pre-processing and variable selection, including partial least squares (PLS), interval partial least squares (iPLS), genetic algorithm (GA) and successive projections algorithm (SPA). Variable selection yielded good results for the correlation coefficient and Root Mean Square Error of Prediction (RMSEP) values for the three parameters, especially triglycerides. The RMSEP values for glucose, triglycerides and HDL produced by the PLS model were 6.08, 16.07 and 2.03 mg dl−1, respectively. F tests and t-tests were performed to compare the results of the models with each other and with a reference method. These results suggests that the PLS method can be used to simultaneously determine the concentrations of glucose, triglycerides and HDL in complicated biological fluids with NIR spectroscopy, offering an alternative analysis in animals.pt_BR
dc.identifier.citationARAUJO, Aurigena Antunes de; et al. Near infrared spectroscopy and multivariate calibration for simultaneous determination of glucose, triglycerides and high-density lipoprotein in animal plasma. Journal of Pharmaceutical and Biomedical Analysis, v. 66, p. 252-257, 2012. Disponível em: <https://www.sciencedirect.com/science/article/pii/S073170851200146X > Acesso em: 13 mar. 2018.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.jpba.2012.03.023
dc.identifier.issn0731-7085
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/25412
dc.languageporpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectNIRpt_BR
dc.subjectGlucosept_BR
dc.subjectTriglyceridespt_BR
dc.subjectHigh-density lipoproteinpt_BR
dc.subjectMultivariate calibrationpt_BR
dc.titleNear infrared spectroscopy and multivariate calibration for simultaneous determination of glucose, triglycerides and high-density lipoprotein in animal plasmapt_BR
dc.typearticlept_BR

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