Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

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dc.contributor.authorAraújo, Aurigena Antunes de
dc.contributor.authorAraújo Júnior, Raimundo Fernandes de
dc.contributor.authorSoares, Luiz Alberto Lira
dc.contributor.authorPorto, Cínthia Raquel da Costa
dc.contributor.authorAquino, Ranniere Gurgel Furtado de
dc.contributor.authorGuedes, Hugo Gonçalo
dc.contributor.authorPetrovick, Pedro Ros
dc.contributor.authorSouza, Tatiane Pereira de
dc.contributor.authorGuerra, Gerlane Coelho Bernardo
dc.date.accessioned2018-06-16T11:29:20Z
dc.date.available2018-06-16T11:29:20Z
dc.date.issued2012-08-21
dc.description.resumoAIM: To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines. METHODS: Colorectal carcinoma (HT29) and human hepatocellular carcinoma (HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri (SDEPN) either alone or in combination with cisplatin at different concentrations (0.5 mg/mL and 1 mg/mL) for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with propidium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms. Significant differences between groups were determined using analysis of variance and Bonferroni’s test, as indicated. A value of P < 0.05 was considered to be statistically significant. RESULTS: SDEPN had significantly different cytotoxic effects on HT29 (2.81 ± 0.11 vs 3.51 ± 1.13, P > 0.05) and HepG2 (5.07 ± 0.3 vs 15.9 ± 1.04, P < 0.001) cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 (1.91 ± 0.57 vs 4.53 ± 1.22, P > 0.05) and HepG2 (14.56 ± 1.6 vs 35.67 ± 3.94, P < 0.001) cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells (HepG2 cells for 4 h: 10.78 ± 1.58 vs 53.89 ± 1.53, P < 0.001; 24 h: 8.9 ± 1.43 vs 62.78 ± 1.87, P < 0.001 and HT29 cells for 4 h: 9.52 ± 0.913 vs 49.86 ± 2.89, P < 0.001; 24 h: 11.78 ± 1.05 vs 53.34 ± 2.65, P < 0.001). In HT29 cells, pretreatment with SDEPN and subsequent treatment with cisplatin resulted in a greater number of cells being killed (12.78 ± 1.01 vs 93.76 ± 1.6, P < 0.001). HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin (12.87 ± 2.78 vs 78.8 ± 3.02, P < 0.001). CONCLUSION: SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells.pt_BR
dc.identifier.citationARAÚJO, Aurigena Antunes de et al. Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines. World Journal of Gastroenterology, v. 18, p. 4162-4168, 2012. Disponível em: <https://www.wjgnet.com/1007-9327/full/v18/i31/4162.htm>. Acesso em: 14 mar. 2018.pt_BR
dc.identifier.doihttp://dx.doi.org/10.3748/wjg.v18.i31.4162
dc.identifier.issn2219-2840
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/25413
dc.languageporpt_BR
dc.publisherBaishidengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectCisplatinpt_BR
dc.subjectColorectal cancerpt_BR
dc.subjectLiver cancerpt_BR
dc.subjectPhyllanthus niruript_BR
dc.subjectCytotoxic effectpt_BR
dc.titleGrowth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell linespt_BR
dc.typearticlept_BR

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