Validation of a novel ELISA for measurement of electronegative low-density lipoprotein

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dc.contributor.authorEvangelista, Karine Cavalcanti Maurício Sena
dc.contributor.authorFaulin, Tanize do Espírito Santo
dc.contributor.authorTelles, Andréia Elisa Rodrigues
dc.contributor.authorGrosso, Daniela de Mattos
dc.contributor.authorFaulin, Edson José Bernardi
dc.contributor.authorAbdalla, Dulcineia Saes Parra
dc.date.accessioned2024-08-26T23:14:32Z
dc.date.available2024-08-26T23:14:32Z
dc.date.issued2008-01
dc.description.resumoBackground: Oxidative modification of low-density lipoprotein (LDL) plays a key role in the pathogenesis of atherosclerosis. LDL(–) is present in blood plasma of healthy subjects and at higher concentrations in diseases with high cardiovascular risk, such as familial hypercholesterolemia or diabetes. Methods: We developed and validated a sandwich ELISA for LDL(–) in human plasma using two monoclonal antibodies against LDL(–) that do not bind to native LDL, extensively copper-oxidized LDL or malondialdehyde-modified LDL. The characteristics of assay performance, such as limits of detection and quantification, accuracy, inter- and intra-assay precision were evaluated. The linearity, interferences and stability tests were also performed. Results: The calibration range of the assay is 0.625–20.0 mU/L at 1:2000 sample dilution. ELISA validation showed intra- and inter-assay precision and recovery within the required limits for immunoassays. The limits of detection and quantification were 0.423 mU/L and 0.517 mU/L LDL(–), respectively. The intra- and inter-assay coefficient of variation ranged from 9.5% to 11.5% and from 11.3% to 18.9%, respectively. Recovery of LDL(–) ranged from 92.8% to 105.1%. Conclusions: This ELISA represents a very practical tool for measuring LDL(–) in human blood for widespread research and clinical sample usept_BR
dc.identifier.citationFAULIN, Tanize do Espírito Santo; EVANGELISTA, Karine Cavalcanti Maurício de Sena; TELLES, Andréia Elisa Rodrigues; GROSSO, Daniela de Mattos; FAULIN, Edson José Bernardi; ABDALLA, Dulcineia Saes Parra. Validation of a novel ELISA for measurement of electronegative low-density lipoprotein. Clinical Chemistry and Laboratory Medicine, [S.l.], v. 46, n. 12, p. 1769-1775, 1 jan. 2008. DOI: 10.1515/cclm.2008.333. Disponível em: https://www.degruyter.com/document/doi/10.1515/CCLM.2008.333/html. Acesso em: 26 jul. 2024.pt_BR
dc.identifier.doihttp://dx.doi.org/10.1515/CCLM.2008.333
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/59902
dc.languageenpt_BR
dc.publisherClinical Chemistry and Laboratory Medicinept_BR
dc.subjectAtherosclerosispt_BR
dc.subjectImmunoassaypt_BR
dc.subjectMethod validationpt_BR
dc.subjectMinimally modified low-density lipoproteinpt_BR
dc.subjectMonoclonal antibodiespt_BR
dc.titleValidation of a novel ELISA for measurement of electronegative low-density lipoproteinpt_BR
dc.typearticlept_BR

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