Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity

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dc.contributor.authorMaciel, Bruna Leal Lima
dc.contributor.authorMedeiros, Amanda Fernandes de
dc.contributor.authorCosta, Izael de Sousa
dc.contributor.authorCarvalho, Fabiana Maria Coimbra de
dc.contributor.authorKiyota, Sumika
dc.contributor.authorSouza, Beatriz Blenda Pinheiro de
dc.contributor.authorSifuentes, Daniel Nogoceke
dc.contributor.authorSerquiz, Raphael Paschoal
dc.contributor.authorUchôa, Adriana Ferreira
dc.contributor.authorSantos, Elizeu Antunes dos
dc.contributor.authorMorais, Ana Heloneida de Araújo
dc.date.accessioned2024-02-01T16:00:58Z
dc.date.available2024-02-01T16:00:58Z
dc.date.issued2018-01
dc.description.resumoA trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M þ 14H]þ ¼ 19,594,690 Da and [M þ 13H]þ ¼ 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 1010mol.L1 and Ki was 2.9 1011mol.L1 . The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related applicationpt_BR
dc.identifier.citationMEDEIROS, Amanda Fernandes de; COSTA, Izael de Sousa; CARVALHO, Fabiana Maria Coimbra de; KIYOTA, Sumika; SOUZA, Beatriz Blenda Pinheiro de; SIFUENTES, Daniel Nogoceke; SERQUIZ, Raphael Paschoal; MACIEL, Bruna Leal Lima; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos; MORAIS, Ana Heloneida de Araújo. Biochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity. Journal Of Enzyme Inhibition And Medicinal Chemistry, [S.l.], v. 33, n. 1, p. 334-348, 1 jan. 2018. DOI: 10.1080/14756366.2017.1419220. Disponível em: https://www.tandfonline.com/doi/full/10.1080/14756366.2017.1419220. Acesso em: 30 jan. 2024.pt_BR
dc.identifier.doihttp://dx.doi.org/10.1080/14756366.2017.1419220
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/57470
dc.languageenpt_BR
dc.publisherJournal of Enzyme Inhibition and Medicinal Chemistrypt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectTamarindpt_BR
dc.subjectAntitrypticpt_BR
dc.subjectWistar ratspt_BR
dc.subjectCCKpt_BR
dc.titleBiochemical characterisation of a kunitz-type inhibitor from tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesitypt_BR
dc.typearticlept_BR

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