CvL, a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotes

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dc.contributor.authorQueiroz, Alexandre F.S.
dc.contributor.authorMoura, Raniere M.
dc.contributor.authorFook, Jacy M.S.L.L.
dc.contributor.authorDias, Anny S.F.
dc.contributor.authorMonteiro, Norberto K.V.
dc.contributor.authorRibeiro, Jannisson K.C.
dc.contributor.authorMoura, Gioconda E.D.D.
dc.contributor.authorMacedo, Leonardo L.P.
dc.contributor.authorSantos, Elizeu A.
dc.contributor.authorSales, Maurício P.
dc.date.accessioned2018-05-28T18:10:39Z
dc.date.available2018-05-28T18:10:39Z
dc.date.issued2006
dc.description.resumoCvL, a lectin from the marine sponge Cliona varians was purified by acetone fractionation followed by Sepharose CL 4B affinity chromatography. CvL agglutinated papainized treated human erythrocytes with preference for type A erythrocytes. The lectin was strongly inhibited by monosaccharide D-galactose and disaccharide sucrose. CvL is a tetrameric glycoprotein of 28 kDa subunits linked by disulphide bridges with a molecular mass of 106 kDa by SDS-PAGE and 114 kDa by Sephacryl S300 gel filtration. The lectin was Ca2+ dependent, stable up to 60 °C for 60 min, with optimum pH of 7.5. CvL displays a cytotoxic effect on gram positive bacteria, such as Bacillus subtilis and Staphylococcus aureus. However, CvL did not affect gram negative bacteria, such as Escherichia coli and Pseudomonas aeruginosa. Leishmania chagasi promastigotes were agglutinated by CvL up to 28 titer. These findings are indicative of the physiological defense roles of CvL and its possible use in the antibiosis of bacteria and protozoa pathogenic.pt_BR
dc.identifier.citationQueiroz,Alexandre F.S.; Moura,Raniere M.; Fook,Jacy M.S.L.L.; Dias,Anny S.F.; Monteiro,Norberto K.V.; Ribeiro,Jannisson K.C.; Moura,Gioconda E.D.D.; Macedo,Leonardo L.P.; Santos, Elizeu A.; Sales,Maurício P. CvL a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotes. Comparative Biochemistry and Physiology. A, Molecular & Integrative Physiology, v. 145, p. 517-523, 2006. Disponível em: <https://www.sciencedirect.com/science/article/pii/S1095643306003692>. Acesso em: 02 mar. 2018. 10.1016/j.cbpa.2006.08.028pt_BR
dc.identifier.doi10.1016/j.cbpa.2006.08.028
dc.identifier.issn0010-406X
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/25251
dc.languageporpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectSpongept_BR
dc.subjectCliona varianspt_BR
dc.subjectPathogenic bacteriapt_BR
dc.subjectLeishmaniapt_BR
dc.subjectInvertebrate marinept_BR
dc.titleCvL, a lectin from the marine sponge Cliona varians: Isolation, characterization and its effects on pathogenic bacteria and Leishmania promastigotespt_BR
dc.typearticlept_BR

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