Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571

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dc.contributor.authorCoghetto, Chaline C.
dc.contributor.authorScherer, Robison P.
dc.contributor.authorSilva, Marceli F.
dc.contributor.authorGolunski, Simone
dc.contributor.authorPergher, Sibele Berenice Castellã
dc.contributor.authorOliveira, Débora de
dc.contributor.authorOliveira, J. Vladimir
dc.contributor.authorTreichel, Helen
dc.date.accessioned2020-06-09T01:47:09Z
dc.date.available2020-06-09T01:47:09Z
dc.date.issued2012-10
dc.description.resumoThe objective of this study was to investigate the process of immobilization of inulinases using natural montmorillonite as inorganic support. The enzyme to buffer ratio of 3:10 and 10 min of immobilization led to the highest specific activity, 375.07 U/mg protein. The immobilized inulinase kept its activity after 1968 h under storage at low temperatures and after 456–1826 h at high temperatures. The pH value of 3.5 led to the highest specific activity. Km values of 1.46 and 0.38 mM, and vmax of 0.2487 and 0.2396 mol/L min, were obtained, respectively, for sucrose and inulin.pt_BR
dc.identifier.citationCOGHETTO, Chaline C.; SCHERER, Robison P.; SILVA, Marceli F.; GOLUNSKI, Simone; PERGHER, Sibele Berenice Castellã; OLIVEIRA, Débora de; OLIVEIRA, J. Vladimir; TREICHEL, Helen. Natural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. Biocatalysis And Agricultural Biotechnology, [s. l.], v. 1, n. 4, p. 284-289, out. 2012. ISSN 1878-8181. DOI https://doi.org/10.1016/j.bcab.2012.06.005. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1878818112001028. Acesso em: 08 jun. 2020.pt_BR
dc.identifier.doi10.1016/j.bcab.2012.06.005
dc.identifier.issn1878-8181
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/29187
dc.languageenpt_BR
dc.publisherElsevierpt_BR
dc.subjectKluyveromyces marxianus NRRL Y-7571pt_BR
dc.subjectInulinasept_BR
dc.subjectInorganic supportpt_BR
dc.subjectImmobilizationpt_BR
dc.titleNatural montmorillonite as support for the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571pt_BR
dc.typearticlept_BR

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