A heterotrimeric GProtein, G«i-3 , on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PKI epithelial cells

dc.contributor.authorAlmeida, Jose Bruno de
dc.contributor.authorStow, Jennifer L.
dc.contributor.authorNarula, Navneet
dc.contributor.authorHoltzman, Eliezer J.
dc.contributor.authorErcolani, Louis
dc.contributor.authorAusiello, Dennis A.
dc.date.accessioned2023-08-03T20:28:25Z
dc.date.available2023-08-03T20:28:25Z
dc.date.issued1991
dc.description.resumoA heterotrimeric Gai subunit, «i- 3 , is localized on Golgi membranes in LLC-PKI and NRK epithelial cells where it colocalizes with mannosidase II by immunofluorescence . The M-3 was found to be localized on the cytoplasmic face of Golgi cisternae and it was distributed across the whole Golgi stack. The a;_3 subunit is found on isolated rat liver Golgi membranes by Western blotting and Gai -3 on the Golgi apparatus is ADP ribosylated by pertussis toxin. LLCPKI cells were stably transfected with GO;-3 on an MT1, inducible promoter in order to overexpress ai-3 on Golgi membranes. The intracellular processing and constitutive secretion of the basement membrane heparan sulfate proteoglycan (HSPG) was measured in LLC-PK, cells. Overexpression of ai-3 on Golgi membranes in transfected cells retarded the secretion of HSPG and accumulated precursors in the medial-trans-Golgi . This effect was reversed by treatment of cells with pertussis toxin which results in ADP-ribosylation and functional uncoupling of Gai-3 on Golgi membranes. These results provide evidence for a novel role for the pertussis toxin sensitive Gai-3 protein in Golgi trafficking of a constitutively secreted protein in epithelial cellspt_BR
dc.identifier.citationALMEIDA, Jose Bruno de; STOW, J L; NARULA, N; HOLTZMAN, E J; ERCOLANI, L; A AUSIELLO, D. A heterotrimeric G protein, G alpha i-3, on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PK1 epithelial cells. The Journal Of Cell Biology, [S.L.], v. 114, n. 6, p. 1113-1124, 15 set. 1991. Rockefeller University Press. http://dx.doi.org/10.1083/jcb.114.6.1113. Disponível em: https://rupress.org/jcb/article/114/6/1113/14234/A-heterotrimeric-G-protein-G-alpha-i-3-on-Golgi. Acesso em: 01 ago. 2023.pt_BR
dc.identifier.doihttps://doi.org/10.1083/jcb.114.6.1113
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/54389
dc.languageenpt_BR
dc.publisherThe Journal Of Cell Biologypt_BR
dc.subjectheterotrimeric G proteinpt_BR
dc.subjectgolgipt_BR
dc.titleA heterotrimeric GProtein, G«i-3 , on Golgi membranes regulates the secretion of a heparan sulfate proteoglycan in LLC-PKI epithelial cellspt_BR
dc.typearticlept_BR

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