A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells

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dc.contributor.authorMorais, Ana Heloneida de Araújo
dc.contributor.authorRabelo, Luciana
dc.contributor.authorMonteiro, Norberto de Kássio Vieira
dc.contributor.authorSerquiz, Raphael Paschoal
dc.contributor.authorSantos, Paula
dc.contributor.authorOliveira, Ruth
dc.contributor.authorOliveira, Adeliana Silva de
dc.contributor.authorRocha, Hugo
dc.contributor.authorUchôa, Adriana Ferreira
dc.contributor.authorSantos, Elizeu Antunes dos
dc.date.accessioned2024-04-12T21:25:01Z
dc.date.available2024-04-12T21:25:01Z
dc.date.issued2012-03
dc.description.resumoCancer represents a set of more than 100 diseases, including malignant tumors from different locations. Strategies inducing differentiation have had limited success in the treatment of established cancers. Marine sponges are a biological reservoir of bioactive molecules, especially lectins. Several animal and plant lectins were purified with antitumor activity, mitogenic, anti-inflammatory and antiviral, but there are few reports in the literature describing the mechanism of action of lectins purified from marine sponges to induce apoptosis in human tumor cells. In this work, a lectin purified from the marine sponge Cinachyrella apion (CaL) was evaluated with respect to its hemolytic, cytotoxic and antiproliferative properties, besides the ability to induce cell death in tumor cells. The antiproliferative activity of CaL was tested against HeLa, PC3 and 3T3 cell lines, with highest growth inhibition for HeLa, reducing cell growth at a dose dependent manner (0.5–10 µg/mL). Hemolytic activity and toxicity against peripheral blood cells were tested using the concentration of IC50 (10 µg/mL) for both trials and twice the IC50 for analysis in flow cytometry, indicating that CaL is not toxic to these cells. To assess the mechanism of cell death caused by CaL in HeLa cells, we performed flow cytometry and western blotting. Results showed that lectin probably induces cell death by apoptosis activation by pro-apoptotic protein Bax, promoting mitochondrial membrane permeabilization, cell cycle arrest in S phase and acting as both dependent and/or independent of caspases pathway. These results indicate the potential of CaL in studies of medicine for treating cancerpt_BR
dc.identifier.citationRABELO, Luciana; MONTEIRO, Norberto de Kássio Vieira; SERQUIZ, Raphael Paschoal; SANTOS, Paula; OLIVEIRA, Ruth; OLIVEIRA, Adeliana Silva de; ROCHA, Hugo; MORAIS, Ana Heloneida de Araújo; UCHÔA, Adriana Ferreira; SANTOS, Elizeu Antunes dos. A lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cells. Marine Drugs, [S.l.], v. 10, n. 12, p. 727-743, 28 mar. 2012. DOI: 10.3390/md10040727. Disponível em: https://www.mdpi.com/1660-3397/10/4/727. Acesso em: 11 abr. 2024.pt_BR
dc.identifier.doihttp://dx.doi.org/10.3390/md10040727
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/58138
dc.languageenpt_BR
dc.publisherMarine Drugspt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectHeLapt_BR
dc.subjectLectinpt_BR
dc.subjectAntitumorpt_BR
dc.subjectMarine spongept_BR
dc.subjectCinachyrella apionpt_BR
dc.titleA lactose-binding lectin from the marine sponge cinachyrella apion (Cal) induces cell death in uuman cervical adenocarcinoma cellspt_BR
dc.typearticlept_BR

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