Preparative chromatography of xylanase using expanded bed adsorption
| dc.contributor.author | Santos, Everaldo Silvino dos | |
| dc.contributor.author | Guirardello, Reginaldo | |
| dc.contributor.author | Franco, Telma Teixeira | |
| dc.date.accessioned | 2021-06-14T14:57:15Z | |
| dc.date.available | 2021-06-14T14:57:15Z | |
| dc.date.issued | 2002-01 | |
| dc.description.resumo | Expanded bed adsorption was used to purify a marketable xylanase often used in the kraft pulp bleaching process. Experiments in packed and expanded beds were carried out mainly to study the adsorption of xylanase on to a cationic adsorbent (Streamline SP) in the presence of cells. In order to study the presence of cells, a Bacillus pumilus mass (5% wet mass) was mixed with the enzyme extract and submitted to an expanded bed adsorption system. One xylanase was purified to homogeneity in the packed bed. However, the 5% cell content hampered purification | pt_BR |
| dc.identifier.citation | SANTOS, E. S.; GUIRARDELLO, R.; FRANCO, T. T.. Preparative chromatography of xylanase using expanded bed adsorption. Journal of Chromatography (Print), Nova Iorque, v. 944, n.1-2, p. 217-224, 2002. Disponível em: https://www.sciencedirect.com/science/article/pii/S0021967301013681?via%3Dihub. Acesso em: 20 abr. 2021. https://doi.org/10.1016/S0021-9673(01)01368-1 | pt_BR |
| dc.identifier.doi | 10.1016/S0021-9673(01)01368-1 | |
| dc.identifier.issn | 0021-9673 | |
| dc.identifier.uri | https://repositorio.ufrn.br/handle/123456789/32673 | |
| dc.language | en | pt_BR |
| dc.publisher | Elsevier | pt_BR |
| dc.rights | Attribution 3.0 Brazil | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/br/ | * |
| dc.subject | Kraft pulp | pt_BR |
| dc.subject | Expanded bed adsorption | pt_BR |
| dc.subject | Adsorption | pt_BR |
| dc.subject | Preparative chromatography | pt_BR |
| dc.subject | Xylanase | pt_BR |
| dc.subject | Enzymes | pt_BR |
| dc.title | Preparative chromatography of xylanase using expanded bed adsorption | pt_BR |
| dc.type | article | pt_BR |
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