Mathematical modeling of the whole expanded bed adsorption process to recover and purify chitosanases from the unclarified fermentation broth of paenibacillus ehimensis

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dc.contributor.authorSouza, Domingos Fabiano de Santana
dc.contributor.authorPadilha, Carlos Eduardo de Araújo
dc.contributor.authorDantas, Paulo Victor Fortunato
dc.contributor.authorSousa Junior, Francisco Canindé de
dc.contributor.authorOliveira, Jackson Araújo de
dc.contributor.authorMacedo, Gorete Ribeiro de
dc.contributor.authorSantos, Everaldo Silvino dos
dc.date.accessioned2021-12-06T18:27:08Z
dc.date.available2021-12-06T18:27:08Z
dc.date.issued2016-12
dc.description.resumoIn this study, a general rate model was applied to the entire process of expanded bed adsorption chromatography (EBAC) for the chitosanases purification protocol from unclarified fermentation broth produced by Paenibacillus ehimensis using the anionic adsorbent Streamline® DEAE. For the experiments performed using the expanded bed, a homemade column (2.6 cm × 30.0 cm) was specially designed. The proposed model predicted the entire EBA process adequately, giving R2 values higher than 0.85 and 2 as low as 0.351 for the elution step. Using the validated model, a 33 factorial design was used to investigate other non-tested conditions as input. It was observed that the superficial velocity during loading and washing steps, as well as the settled bed height, has a strong positive effect on the F objective function used to evaluate the production of the purified chitosanasespt_BR
dc.identifier.citationPADILHA, Carlos Eduardo de Araújo; DANTAS, Paulo Victor Fortunato; SOUSA, Francisco Canindé de; SOUZA, Domingos Fabiano de Santana; OLIVEIRA, Jackson Araújo de; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Mathematical modeling of the whole expanded bed adsorption process to recover and purify chitosanases from the unclarified fermentation broth of Paenibacillus ehimensis. Journal Of Chromatography B, [S.L.], v. 1039, p. 44-50, dez. 2016. Elsevier BV. http://dx.doi.org/10.1016/j.jchromb.2016.10.027. Disponível em: https://www.sciencedirect.com/science/article/pii/S1570023216311084?via%3Dihub. Acesso em: 05 nov. 2021pt_BR
dc.identifier.doi10.1016/j.jchromb.2016.10.027
dc.identifier.issn1570-0232
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/45194
dc.languageenpt_BR
dc.publisherElsevierpt_BR
dc.subjectMathematical modelingpt_BR
dc.subjectExpanded bed adsorptionpt_BR
dc.subjectChitosanasespt_BR
dc.subjectFermentation brothpt_BR
dc.subjectExperimental designpt_BR
dc.titleMathematical modeling of the whole expanded bed adsorption process to recover and purify chitosanases from the unclarified fermentation broth of paenibacillus ehimensispt_BR
dc.typearticlept_BR

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