Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus

Moura, Daniela Maria de Sousa; Brandão, Juliana Alves; Lentini, Celia; Heinrich, Christophe; Queiroz, Claudio Marcos Teixeira de; Costa, Marcos Romualdo

Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus

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dc.contributor.author	Moura, Daniela Maria de Sousa
dc.contributor.author	Brandão, Juliana Alves
dc.contributor.author	Lentini, Celia
dc.contributor.author	Heinrich, Christophe
dc.contributor.author	Queiroz, Claudio Marcos Teixeira de
dc.contributor.author	Costa, Marcos Romualdo
dc.date.accessioned	2020-09-18T14:32:13Z
dc.date.available	2020-09-18T14:32:13Z
dc.date.issued	2020-09-18
dc.description.resumo	Cell lineage in the adult hippocampus comprises multipotent and neuron-committed progenitors. In the present work, we fate-mapped neuronal progenitors using Dcx-CreERT2 and CAG-CAT-EGFP double-transgenic mice (cDCX/EGFP). We show that 3 days after tamoxifen-mediated recombination in cDCX/EGFP adult mice, GFP+ cells in the dentate gyrus (DG) co-expresses DCX and about 6% of these cells are proliferative neuronal progenitors. After 30 days, 20% of GFP+ generated from these progenitors differentiate into GFAP+ astrocytes. Unilateral intrahippocampal administration of the chemoconvulsants kainic acid (KA) or pilocarpine (PL) triggered epileptiform discharges and led to a significant increase in the number of GFP+ cells in both ipsi and contralateral DG. However, while PL favored the differentiation of neurons in both ipsi- and contralateral sides, KA stimulated neurogenesis only in the contralateral side. In the ipsilateral side, KA injection led to an unexpected increase of astrogliogenesis in the Dcx-lineage. We also observed a small number of GFP+/GFAP+ cells displaying radial-glia morphology ipsilaterally 3 days after KA administration, suggesting that some Dcx-progenitors could regress to a multipotent stage. The boosted neurogenesis and astrogliogenesis observed in the Dcx-lineage following chemoconvulsants administration correlated, respectively, with preservation or degeneration of the parvalbuminergic plexus in the DG. Increased inflammatory response, by contrast, was observed both in the DG showing increased neurogenesis or astrogliogenesis. Altogether, our data support the view that cell lineage progression in the adult hippocampus is not unidirectional and could be modulated by local network activity and GABA-mediated signaling	pt_BR
dc.identifier.citation	MOURA, D. M. S.; BRANDÃO, J. A.; LENTINI, C.; HEINRICH, C.; QUEIROZ, C. M.; COSTA, M. R. Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus. Front. Neurosci., [S. L.], v. 14, p. 571315, set. 2020. doi: 10.3389/fnins.2020.571315. Disponível em: https://www.frontiersin.org/articles/10.3389/fnins.2020.571315/full. Acesso em: 18 set. 2020.	pt_BR
dc.identifier.doi	10.3389/fnins.2020.571315
dc.identifier.uri	https://repositorio.ufrn.br/jspui/handle/123456789/30098
dc.language	en	pt_BR
dc.publisher	Frontiers in Neuroscience	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Adult hippocampus	pt_BR
dc.subject	Neurogenesis	pt_BR
dc.subject	Astrogliogenesis	pt_BR
dc.subject	Status epilepticus	pt_BR
dc.subject	Fate-specification	pt_BR
dc.subject	Kainic acid	pt_BR
dc.subject	Pilocarpine	pt_BR
dc.subject	GABAergic interneurons	pt_BR
dc.title	Evidence of progenitor cell lineage rerouting in the adult mouse hippocampus after status epilepticus	pt_BR
dc.type	article	pt_BR