Quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone–water extracts of Libidibia ferrea, Parapiptadenia rigida and Psidium guajava

Página do item simplificado Estatísticas
dc.contributor.authorAraújo, Aurigena Antunes de
dc.contributor.authorSoares, Luiz Alberto Lira
dc.contributor.authorFerreira, Magda Rhayanny Assunção
dc.contributor.authorSouza Neto, Manoel Andréde
dc.contributor.authorSilva, Giselle Ribeiroda
dc.contributor.authorAraújo Jr., Raimundo Fernandes de
dc.contributor.authorGuerra, Gerlane Coelho Bernardo
dc.contributor.authorMelo, Maria Celeste Nunes de
dc.date.accessioned2018-06-16T11:47:23Z
dc.date.available2018-06-16T11:47:23Z
dc.date.issued2014
dc.description.resumoEthnopharmacological relevance Vast numbers of plant species from northeastern Brazil have not yet been phytochemically or biologically evaluated. Aim of the study The goal of this work was to obtain, characterize and show the antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone–water extracts of Libidibia ferrea, Parapiptadenia rigida and Psidium guajava. Materials and methods The plant material (100 g) was dried, and the crude extracts were obtained by using turbo-extraction (10%; w/v) with water or acetone:water (7:3, v/v) as the extraction solvent. High-performance liquid chromatography (HPLC) methods were used to screen the crude extracts for hydrolysable tannins (gallic acid) and condensed tannins (catechins). The antibacterial activity was evaluated by agar-diffusion and microdilution methods against Gram-positive strains (Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis INCQS 00016, Enterococcus faecalis ATCC 29212 and a clinical isolate of methicillin-resistant Staphylococcus aureus) as well as Gram-negative strains (Escherichia coli ATCC 25922, Salmonella enteritidis INCQS 00258, Shigella flexneri and Klebsiella pneumoniae). To evaluate the anti-inflammatory activity, a leukocyte migration model was used. Analgesic activity was determined by the hot plate test and the acetic acid-induced abdominal writhing test. Data were analyzed by analysis of variance (ANOVA) at a significance level of 5%. Results Parapiptadenia rigida presented the highest amount of total polyphenols (35.82±0.20%), while the greatest catechin content was found in the acetone–water extract of Psidium guajava (EAWPg; 1.04 μg/g). The largest amounts of catechins were found in the aqueous extract of Libidibia ferrea (EALf; 1.07 μg/g) and the acetone–water extract of Parapiptadenia rigida (EAWPr; 1.0 μg/g). All extracts showed activity against Gram-positive bacteria. The aqueous and acetone–water extracts of Psidium guajava showed the greatest inhibition zones in the agar diffusion tests. In the evaluation of the minimum inhibitory concentration (MIC), the most susceptible Gram-positive bacterium was Staphylococcus epidermidis and the most susceptible Gram-negative bacterium was Shigella flexneri. EAPg and EAWPg showed the greatest MIC values. All extracts were significant inhibitors of leukocyte migration (p<0.05). Using the writhing test, significant analgesic activity was found for EAPr (50 mg/kg), EAWPr (100 mg/kg and 200 mg/kg) and EAWPg (50 mg/kg) (p<0.05). Conclusions Thus, the appropriate extraction procedure preserves the chemical components such as gallic acid and catechin, and showed antimicrobial, anti-inflammatory and analgesic properties.pt_BR
dc.identifier.citationARAÚJO, Aurigena Antunes de et al. Quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone-water extracts of Libidibia ferrea, Parapiptadenia rigida and Psidium guajava. Journal of Ethnopharmacology, v. 156, p. 88-96, 2014. Disponível em: <https://www.sciencedirect.com/science/article/pii/S0378874114005509>. Acesso em: 19 mar. 2018.pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.jep.2014.07.031
dc.identifier.issn0378-8741
dc.identifier.urihttps://repositorio.ufrn.br/jspui/handle/123456789/25420
dc.languageengpt_BR
dc.publisherElsevierpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectLibidibia ferreapt_BR
dc.subjectParapiptadeniarigidapt_BR
dc.subjectPsidium guajavapt_BR
dc.subjectPolyphenolspt_BR
dc.subjectAntimicrobialpt_BR
dc.subjectAntiinflammatorypt_BR
dc.titleQuantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone–water extracts of Libidibia ferrea, Parapiptadenia rigida and Psidium guajavapt_BR
dc.typearticlept_BR

Arquivos

Pacote Original

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
QuantificationPolyphenolsEvaluation_Araujo_2014.pdf
Tamanho:
770.46 KB
Formato:
Adobe Portable Document Format
Nenhuma Miniatura disponível
Baixar

Licença do Pacote

Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Nenhuma Miniatura disponível
Baixar

Coleções

CB - DBF - Artigos publicados em periódicos
Repositório Institucional - UFRN Campus Universitário Lagoa NovaCEP 59078-970 Caixa postal 1524 Natal/RN - BrasilUniversidade Federal do Rio Grande do Norte© Copyright 2025. Todos os direitos reservados.
Contato+55 (84) 3342-2260 - R232Setor de Repositórios Digitaisrepositorio@bczm.ufrn.br
DSpaceIBICT
OasisBR
LAReferencia
Customizado pela CAT - BCZM