Zebrafish automatic monitoring system for conditioning and behavioral analysis

Barreiros, Marta de Oliveira; Barbosa, Felipe Gomes; Dantas, Diego de Oliveira; Santos, Daniel de Matos Luna dos; Ribeiro, Sidarta Tollendal Gomes; Santos, Giselle Cutrim de Oliveira; Barros, Allan Kardec

Zebrafish automatic monitoring system for conditioning and behavioral analysis

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dc.contributor.author	Barreiros, Marta de Oliveira
dc.contributor.author	Barbosa, Felipe Gomes
dc.contributor.author	Dantas, Diego de Oliveira
dc.contributor.author	Santos, Daniel de Matos Luna dos
dc.contributor.author	Ribeiro, Sidarta Tollendal Gomes
dc.contributor.author	Santos, Giselle Cutrim de Oliveira
dc.contributor.author	Barros, Allan Kardec
dc.date.accessioned	2021-04-30T13:20:40Z
dc.date.available	2021-04-30T13:20:40Z
dc.date.issued	2021-04-29
dc.description.resumo	Studies using zebrafish (Danio rerio) in neuro-behavioural research are growing. Measuring fish behavior by computational methods is one of the most efficient ways to avoid human bias in experimental analyses, extending them to various approaches. Sometimes, thorough analyses are difficult to do, as fish can behave unpredictably during an experimental strategy. However, the analyses can be implemented in an automated way, using an online strategy and video processing for a complete assessment of the zebrafish behavior, based on the detection and tracking of fish during an activity. Here, a fully automatic conditioning and detailed analysis of zebrafish behavior is presented. Microcontrolled components were used to control the delivery of visual and sound stimuli, in addition to the concise amounts of food after conditioned stimuli for adult zebrafish groups in a conventional tank. The images were captured and processed for automatic detection of the fish, and the training of the fish was done in two evaluation strategies: simple and complex. In simple conditioning, the zebrafish showed significant responses from the second attempt, learning that the conditioned stimulus was a predictor of food presentation in a specific space of the tank, where the food was dumped. When the fish were subjected to two stimuli for decision-making in the food reward, the zebrafish obtained better responses to red light stimuli in relation to vibration. The behavior change was clear in stimulated fish in relation to the control group, thus, the distances traveled and the speed were greater, while the polarization was lower in stimulated fish. This automated system allows for the conditioning and assessment of zebrafish behavior online, with greater stability in experiments, and in the analysis of the behavior of individual fish or fish schools, including learning and memory studies	pt_BR
dc.identifier.citation	BARREIROS, Marta de Oliveira; BARBOSA, Felipe Gomes; DANTAS, Diego de Oliveira; SANTOS, Daniel de Matos Luna dos; RIBEIRO, Sidarta; SANTOS, Giselle Cutrim de Oliveira; BARROS, Allan Kardec. Zebrafish automatic monitoring system for conditioning and behavioral analysis. Scientific Reports, [S.L.], v. 11, p. 9330, abr. 2021. Doi: http://dx.doi.org/10.1038/s41598-021-87502-6. Disponível em: https://www.nature.com/articles/s41598-021-87502-6. Acesso em: 30 abr. 2021.	pt_BR
dc.identifier.doi	10.1038/s41598-021-87502-6
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/32343
dc.language	en	pt_BR
dc.publisher	Springer Science and Business Media LLC	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Zebrafish - Automatic monitoring system	pt_BR
dc.subject	YOLOv2 network	pt_BR
dc.subject	Behavior, animal	pt_BR
dc.title	Zebrafish automatic monitoring system for conditioning and behavioral analysis	pt_BR
dc.type	article	pt_BR