Single-step purification of chitosanases from Bacillus cereus using expanded bed chromatography

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dc.contributor.authorSantos, Everaldo Silvino dos
dc.contributor.authorAraújo, Nathália Kelly de
dc.contributor.authorPagnoncelli, Maria Giovana Binder
dc.contributor.authorPimentel, Vanessa Carvalho
dc.contributor.authorXavier, Maria Luiza Oliveira
dc.contributor.authorPadilha, Carlos Eduardo Araújo
dc.contributor.authorMacedo, Gorete Ribeiro de
dc.date.accessioned2021-05-10T19:26:17Z
dc.date.available2021-05-10T19:26:17Z
dc.date.issued2016-01
dc.description.resumoA chitosanase-producing strain was isolated and identified as Bacillus cereus C-01. The purification and characterization of two chitosanases were studied. The purification assay was accomplished by ion exchange expanded-bed chromatography. Experiments were carried out in the presence and in the absence of cells through different expansion degree to evaluate the process performance. The adsorption experiments demonstrated that the biomass does not affect substantially the adsorption capacity of the matrix. The enzyme bound to the resin with the same extent using clarified and unclarified broth (0.32 and 0.30 U/g adsorbent, respectively). The fraction recovered exhibited 31% of the yield with a 1.26-fold increase on the specific activity concerned to the initial broth. Two chitosanases from different elution steps were recovery. Chit A and Chit B were stable at 30–60 ◦C, pH 5.5–8.0 and 5.5–7.5, respectively. The highest activity was found at 55 ◦C, pH 5.5 to Chit A and 50 ◦C, pH 6.5 to Chit B. The ions Cu2+, Fe2+ and Zn2+ indicated inhibitory effect on chitosanases activities that were significantly activated by Mn2+. The methodology applied in this study enables the partial purification of a stable chitosanase using a feedstock without any pre-treatment using a single-step purificationpt_BR
dc.identifier.citationARAÚJO, Nathália Kelly de; PAGNONCELLI, Maria Giovana Binder; PIMENTEL, Vanessa Carvalho; XAVIER, Maria Luiza Oliveira; PADILHA, Carlos Eduardo Araújo; MACEDO, Gorete Ribeiro de; SANTOS, Everaldo Silvino dos. Single-step purification of chitosanases from Bacillus cereus using expanded bed chromatography. International Journal Of Biological Macromolecules, [S.L.], v. 82, p. 291-298, jan. 2016. Disponivel em https://www.sciencedirect.com/science/article/pii/S0141813015006789?via%3Dihub Acesso em 04 abr. 2021. https://doi.org/10.1016/j.ijbiomac.2015.09.063pt_BR
dc.identifier.doi10.1016/j.ijbiomac.2015.09.063
dc.identifier.issn0141-8130
dc.identifier.urihttps://repositorio.ufrn.br/handle/123456789/32481
dc.languageenpt_BR
dc.publisherElsevierpt_BR
dc.rightsAttribution 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/br/*
dc.subjectChitosanpt_BR
dc.subjectChitosanasept_BR
dc.subjectB.cereuspt_BR
dc.subjectBioseparationpt_BR
dc.subjectIon exchangept_BR
dc.subjectExpanded bed adsorptionpt_BR
dc.titleSingle-step purification of chitosanases from Bacillus cereus using expanded bed chromatographypt_BR
dc.typearticlept_BR

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