Produção de biotensoativo utilizando Pseudomonas aeruginosa (P.A.) e resíduo agroindustrial (manipueira) como substrato

Abstract

Biosurfactants are amphoteric molecules that act preferentially on the interface of fluids of different polarities such as water and oil, and they may be synthesized by micro-organisms. Using a factorial design 24-1 (half fraction) 10 assays were performed using Pseudomonas aeruginosa (PA) AP029/GVII-A as microorganism in rotary incubator (shaker), in which we analyzed the influence of factors (temperature, agitation, rate of aeration and concentration of culture medium) at two different levels for the synthesis of biosurfactant. Samples were collected throughout the cultivation until we completed 132h of fermentation. We tried the best result following the production through consumption of substrate, dry matter, reduction of surface tension (ring method) and emulsification index. The results showed that the cassava wastewater is a very assimilable substrate for the production of biosurfactant, reaching 91% of consumption by the micro-organism under study. The cultivation temperature was found to be one of the leading factors in the synthesis of the metabolite, followed by aeration rate and also due to the agitation. The best results showed a reduction of 30% in surface tension (% RTS) for the medium, reaching values of 30 mN/m, 3.0 g / L of biomass and emulsification index greater than 65%