Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system

Santos, Everaldo Silvino dos; Oliveira Júnior, Sérgio Dantas de; Padilha, Carlos Eduardo de Araújo; Asevedo, Estefani Alves de; Macedo, Gorete Ribeiro de

Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system

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dc.contributor.author	Santos, Everaldo Silvino dos
dc.contributor.author	Oliveira Júnior, Sérgio Dantas de
dc.contributor.author	Padilha, Carlos Eduardo de Araújo
dc.contributor.author	Asevedo, Estefani Alves de
dc.contributor.author	Macedo, Gorete Ribeiro de
dc.date.accessioned	2021-04-26T13:31:43Z
dc.date.available	2021-04-26T13:31:43Z
dc.date.issued	2020-05-04
dc.description.resumo	Purpose: In this study, an aqueous two-phase micellar system (ATPMS), formed by the non-ionic surfactant Triton X-114, was used to investigate the partitioning of cellulolytic enzymes produced by the filamentous fungus Aspergillus fumigatus CCT 7873. Methods: Performance of the ATPMS on the partitioning of CMCase (activity on carboxymethyl cellulose) and FPase (activity on filter paper) was investigated by varying the temperature (35, 40, 45, 50, 55, 60, and 65 °C), enzyme crude extract concentration (20, 40, 60, and 80% w/w), and Triton X-114 concentration (2, 4, 6, and 8% w/w) and by adding different inorganic salts (NaCl, CaCl2, MgSO4, and MnSO4) in the system. Results: An ATPMS formed with 8% (w/w) Triton X-114 and 40% (w/w) enzymatic crude extract at a system temperature of 55 °C was most favorable for partitioning the tested enzymes. Under these conditions, a purification factor for CMCase and FPase of 10.89 and 0.65 was reached, respectively. The addition of inorganic salts changed the distribution of enzymes. Of these, CaCl2 contributed to a higher distribution coefficient (50.0), whereas for FPase, the presence of MnSO4 in the system improved the purification factor to 3.94. Conclusion: The highest values obtained for the yield and purification factors demonstrate that ATPMS is an interesting option for recovering and purifying cellulolytic enzymes	pt_BR
dc.identifier.citation	OLIVEIRA JÚNIOR, S.D.; PADILHA, C.E.A.; ASEVEDO, E.A.; MACEDO, G.R.; SANTOS, E.S.. Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system. Annals of Microbiology, v. 70, p. 23, 2020. Disponível em https://annalsmicrobiology.biomedcentral.com/articles/10.1186/s13213-020-01573-w. Acesso em 30 mar. 2021. https://doi.org/10.1186/s13213-020-01573-w	pt_BR
dc.identifier.doi	10.1186/s13213-020-01573-w
dc.identifier.issn	1590-4261
dc.identifier.issn	1869-2044
dc.identifier.uri	https://repositorio.ufrn.br/handle/123456789/32311
dc.language	en	pt_BR
dc.publisher	Annals of Microbiology	pt_BR
dc.rights	Attribution 3.0 Brazil	*
dc.rights.uri	http://creativecommons.org/licenses/by/3.0/br/	*
dc.subject	Aspergillus fumigatus	pt_BR
dc.subject	Cellulases	pt_BR
dc.subject	Micelles	pt_BR
dc.subject	Liquid-liquid extraction	pt_BR
dc.subject	Downstream processing	pt_BR
dc.title	Recovery and purification of cellulolytic enzymes from Aspergillus fumigatus CCT 7873 using an aqueous two-phase micellar system	pt_BR
dc.type	article	pt_BR